High-risk human papillomavirus infection and p16INK4a protein expression in laryngeal lesions
Language English Country Germany Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
18440161
DOI
10.1016/j.prp.2008.03.001
PII: S0344-0338(08)00071-X
Knihovny.cz E-resources
- MeSH
- Alphapapillomavirus genetics isolation & purification MeSH
- Child MeSH
- DNA Probes, HPV MeSH
- DNA, Viral isolation & purification MeSH
- Adult MeSH
- In Situ Hybridization MeSH
- Immunohistochemistry MeSH
- Papillomavirus Infections complications diagnosis virology MeSH
- Cyclin-Dependent Kinase Inhibitor p16 analysis MeSH
- Carcinoma * chemistry pathology virology MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Laryngeal Neoplasms * chemistry pathology virology MeSH
- Papilloma * chemistry pathology virology MeSH
- Polymerase Chain Reaction MeSH
- Child, Preschool MeSH
- Precancerous Conditions * chemistry pathology virology MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Sensitivity and Specificity MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Male MeSH
- Child, Preschool MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- DNA Probes, HPV MeSH
- DNA, Viral MeSH
- Cyclin-Dependent Kinase Inhibitor p16 MeSH
A total of 88 samples of laryngeal lesions (23 vocal cord nodules (VCNs), 23 papillomas (PAs), 18 dysplasias (DYs), and 24 carcinomas (CAs)) were analyzed for p16INK4a protein (p16) expression by immunohistochemistry and for high-risk human papillomavirus (HR-HPV) infection using chromogene in situ hybridization (CISH) and polymerase chain reaction (PCR). The series comprised 62 males and 26 females, aged 1-87 years (median 55 years). p16 expression was detected in 2 of 23 (9%) VCNs, 18 of 23 (78%) PAs, 9 of 18 (50%) DYs, and 14 of 24 (58%) CAs. Using CISH, HR-HPV DNA was detected in 3 of 23 (13%) VCNs, in 19 of 23 (83%) PAs, in 12 of 18 (67%) DYs, and in 14 of 24 (58%) CAs. HR-HPV DNA was found in six of nine (67%) PAs by PCR. A statistically significant difference in p16 expression and HR-HPV DNA presence detected by CISH was observed between VCNs and PAs (p<0.000001). The sensitivity and specificity of p16 expression for HR-HPV DNA presence detected by CISH was 0.612 and 0.773, respectively. Our study confirms a potential role of HR-HPV infection not only in the pathogenesis of malignant, but also in benign laryngeal lesions.
References provided by Crossref.org
The role of protein p16(INK4a) in glottic laryngeal squamous cell carcinoma
