Quantitative nitric oxide production by rat, bovine and porcine macrophages
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18456005
DOI
10.1016/j.niox.2008.04.001
PII: S1089-8603(08)00044-X
Knihovny.cz E-resources
- MeSH
- Macrophages, Alveolar * metabolism pathology MeSH
- Biological Assay MeSH
- Ethylenediamines MeSH
- Immunohistochemistry MeSH
- Interferon-gamma toxicity MeSH
- Ionomycin toxicity MeSH
- Rats MeSH
- Cells, Cultured MeSH
- Lipopolysaccharides toxicity MeSH
- RNA, Messenger metabolism MeSH
- Nitric Oxide * analysis metabolism MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Swine MeSH
- Gene Expression Regulation, Enzymologic MeSH
- Recombinant Proteins MeSH
- Cattle MeSH
- Sulfanilamides MeSH
- Nitric Oxide Synthase Type II genetics metabolism MeSH
- Tetradecanoylphorbol Acetate toxicity MeSH
- Blotting, Western MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Ethylenediamines MeSH
- Griess reagent MeSH Browser
- Interferon-gamma MeSH
- Ionomycin MeSH
- Lipopolysaccharides MeSH
- RNA, Messenger MeSH
- Nitric Oxide * MeSH
- Recombinant Proteins MeSH
- Sulfanilamides MeSH
- Nitric Oxide Synthase Type II MeSH
- Tetradecanoylphorbol Acetate MeSH
The aim of this work was to compare in vitro nitric oxide (NO) production by rat, bovine and porcine macrophages. NO production was induced by lipopolysaccharide (LPS) or by phorbol 12-myristate 13-acetate (PMA) with ionomycin or recombinant interferon gamma (rIFN-gamma) and was assessed by Griess reaction. NO synthase type II (NOS II) expression was quantified by immunocytochemistry, Western blot and real-time polymerase chain reaction (RT-PCR). There were differences in NO production by pulmonary alveolar macrophages (PAM) in all species tested. The largest amounts of NO were produced by rat PAM. Less NO was produced by bovine PAM. Moreover, PAM in rats and cows differed in their abilities to respond to various stimulators. Neither porcine PAM nor Kupffer cells produced NO. Stimulation of porcine PAM with alternative concentrations of LPS did not lead to inducing NO production. Stimulation of porcine PAM with rIFN-gamma together with LPS led to a significant increase in the expression of NOS II mRNA, albeit without detectable NO production or NOS II expression on the protein level.
References provided by Crossref.org
Bovine lactoferrin free of lipopolysaccharide can induce a proinflammatory response of macrophages
