Cross-coupling reactions of nucleoside triphosphates followed by polymerase incorporation. Construction and applications of base-functionalized nucleic acids
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
18563253
DOI
10.1039/b803664k
Knihovny.cz E-resources
- MeSH
- DNA-Directed RNA Polymerases metabolism MeSH
- DNA-Directed DNA Polymerase metabolism MeSH
- Nucleotides chemistry genetics metabolism MeSH
- Nucleic Acids metabolism MeSH
- Polyphosphates chemistry MeSH
- Base Sequence MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Names of Substances
- DNA-Directed RNA Polymerases MeSH
- DNA-Directed DNA Polymerase MeSH
- Nucleotides MeSH
- Nucleic Acids MeSH
- Polyphosphates MeSH
- triphosphoric acid MeSH Browser
Construction of functionalized nucleic acids (DNA or RNA) via polymerase incorporation of modified nucleoside triphosphates is reviewed and selected applications of the modified nucleic acids are highlighted. The classical multistep approach for the synthesis of modified NTPs by triphosphorylation of modified nucleosides is compared to the novel approach consisting of direct aqueous cross-coupling reactions of unprotected halogenated nucleoside triphosphates. The combination of cross-coupling of NTPs with polymerase incorporation gives an efficient and straightforward two-step synthesis of modified nucleic acids. Primer extension using biotinylated templates followed by separation using streptavidine-coated magnetic beads and DNA duplex denaturation is used for preparation of modified single stranded oligonucleotides. Examples of using this approach for electrochemical DNA labelling and bioanalytical applications are given.
References provided by Crossref.org
Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases
