Cross-coupling reactions of nucleoside triphosphates followed by polymerase incorporation. Construction and applications of base-functionalized nucleic acids
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem, přehledy
PubMed
18563253
DOI
10.1039/b803664k
Knihovny.cz E-zdroje
- MeSH
- DNA řízené RNA-polymerasy metabolismus MeSH
- DNA-dependentní DNA-polymerasy metabolismus MeSH
- nukleotidy chemie genetika metabolismus MeSH
- nukleové kyseliny metabolismus MeSH
- polyfosfáty chemie MeSH
- sekvence nukleotidů MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- Názvy látek
- DNA řízené RNA-polymerasy MeSH
- DNA-dependentní DNA-polymerasy MeSH
- nukleotidy MeSH
- nukleové kyseliny MeSH
- polyfosfáty MeSH
- triphosphoric acid MeSH Prohlížeč
Construction of functionalized nucleic acids (DNA or RNA) via polymerase incorporation of modified nucleoside triphosphates is reviewed and selected applications of the modified nucleic acids are highlighted. The classical multistep approach for the synthesis of modified NTPs by triphosphorylation of modified nucleosides is compared to the novel approach consisting of direct aqueous cross-coupling reactions of unprotected halogenated nucleoside triphosphates. The combination of cross-coupling of NTPs with polymerase incorporation gives an efficient and straightforward two-step synthesis of modified nucleic acids. Primer extension using biotinylated templates followed by separation using streptavidine-coated magnetic beads and DNA duplex denaturation is used for preparation of modified single stranded oligonucleotides. Examples of using this approach for electrochemical DNA labelling and bioanalytical applications are given.
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