High expression of ERCC1, FLT1, NME4 and PCNA associated with poor prognosis and advanced stages in myelodysplastic syndrome
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18604718
DOI
10.1080/10428190802129918
PII: 794748215
Knihovny.cz E-resources
- MeSH
- DNA-Binding Proteins genetics MeSH
- Adult MeSH
- Endonucleases genetics MeSH
- Gene Expression MeSH
- Middle Aged MeSH
- Humans MeSH
- Myelodysplastic Syndromes diagnosis MeSH
- Biomarkers, Tumor analysis MeSH
- Neoplasm Proteins genetics MeSH
- NM23 Nucleoside Diphosphate Kinases genetics MeSH
- Nucleoside Diphosphate Kinase D MeSH
- Prognosis MeSH
- Disease Progression MeSH
- Proliferating Cell Nuclear Antigen genetics MeSH
- Vascular Endothelial Growth Factor Receptor-1 genetics MeSH
- Oligonucleotide Array Sequence Analysis MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Gene Expression Profiling * MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA-Binding Proteins MeSH
- Endonucleases MeSH
- ERCC1 protein, human MeSH Browser
- FLT1 protein, human MeSH Browser
- Biomarkers, Tumor MeSH
- Neoplasm Proteins MeSH
- NM23 Nucleoside Diphosphate Kinases MeSH
- NME4 protein, human MeSH Browser
- Nucleoside Diphosphate Kinase D MeSH
- Proliferating Cell Nuclear Antigen MeSH
- Vascular Endothelial Growth Factor Receptor-1 MeSH
Myelodysplastic syndrome (MDS) represents a good model for research of prognostic/progression markers due to frequent transformation into acute myeloid leukemia (AML). We analysed expression profiles of 26 MDS and 6 AML patients using cDNA arrays comprising 588 gene probes. The array data were validated in a larger set of 46 patients by qRT-PCR. Data analysis identified differently expressed genes in MDS and the cluster of four genes (ERCC1, FLT1, NME4 and PCNA) whose expression was correlated with MDS subtypes. High expression of these genes was associated with poor prognosis and/or unfavorable outcome. Furthermore, PCNA expression was correlated with peripheral blood blast percentage (r = 0.71, p < 0.05), while the other genes showed non-significant correlation. Our findings demonstrate the progressive up-regulation of the genes along the sequence of 5q-syndrome/RCMD/RAEB/de novo AML, suggesting their association with disease progression.
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