Chitinolytic activity of the sheep rumen ciliate Diploplastron affine
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- bachor metabolismus parazitologie MeSH
- chitin metabolismus MeSH
- chitinasy chemie klasifikace metabolismus MeSH
- Ciliophora enzymologie růst a vývoj MeSH
- kultivační média chemie MeSH
- ovce metabolismus parazitologie MeSH
- protozoální proteiny klasifikace metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- chitin MeSH
- chitinasy MeSH
- kultivační média MeSH
- protozoální proteiny MeSH
Supplementation of the rumen ciliate Diploplastron affine growth medium with commercial chitin stimulated growth of ciliates and the density of their population was positively correlated with chitin doses (r = 0.95; p < 0.01). The cell-free extracts prepared from bacteria-free ciliates degraded chitin to N-acetyl-D: -glucosamine and chitobiose. Three exochitinases, two endochitinases and two beta-N-acetylglucosaminidases were identified in the cell-free extract of protozoa. The molar mass of exochitinases was 80, 65 and 30 kDa, and endochitinases 75 and 50 kDa; the molar mass of one of the identified beta-N-acetylglucosaminidases was 45 kDa.
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Nature. 1970 Aug 15;227(5259):680-5 PubMed
J Gen Microbiol. 1972 Dec;73(3):509-21 PubMed
J Bacteriol. 1976 Jan;125(1):324-31 PubMed
Microbiology (Reading). 1994 Mar;140 ( Pt 3):631-6 PubMed
Appl Environ Microbiol. 1999 Dec;65(12):5338-44 PubMed
Anal Biochem. 1993 Jan;208(1):74-9 PubMed
Curr Microbiol. 1996 Mar;32(3):115-8 PubMed
Folia Microbiol (Praha). 2000;45(5):465-8 PubMed
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The ability of the rumen protozoan Eudiplodinium maggii to utilize chitin
Mureinolytic ability of the rumen ciliate Diploplastron affine
