The ability of the rumen protozoan Eudiplodinium maggii to utilize chitin
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- acetylglukosamin metabolismus MeSH
- bachor parazitologie MeSH
- chitin izolace a purifikace metabolismus MeSH
- Ciliophora izolace a purifikace metabolismus MeSH
- houby chemie MeSH
- kultivační média chemie MeSH
- kyselina máselná metabolismus MeSH
- kyselina octová metabolismus MeSH
- kyseliny mastné těkavé metabolismus MeSH
- ovce MeSH
- propionáty metabolismus MeSH
- trisacharidy metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- acetylglukosamin MeSH
- chitin MeSH
- chitotriose MeSH Prohlížeč
- kultivační média MeSH
- kyselina máselná MeSH
- kyselina octová MeSH
- kyseliny mastné těkavé MeSH
- propionáty MeSH
- propionic acid MeSH Prohlížeč
- trisacharidy MeSH
The ability was determined of the rumen ciliate Eudiplodinium maggii to utilize chitin from fungal cell wall. Cultivation experiments shoved that the population concentration (number of ciliates in vitro) was positively correlated with chitin doses. Cell extract prepared from the bacteria-free ciliates degraded colloidal chitin releasing 2.0 micromol reducing sugar per mg protein per h. End products of this reaction were chitotriose and N-acetylglucosamine. Incubation of the bacteria-free ciliates with chitin resulted in an increase in the concentration of acetic, propionic and butyric acids in the incubation medium. The production rate of total volatile fatty acids (VFA) by ciliates incubated with and without chitin was 45.0 and 30.5 pmol VFA per protozoan, respectively, the molar proportion of particular acids remaining unchanged.
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Chitinolytic enzymes of the rumen ciliate Eudiplodinium maggii
