The ability of the rumen protozoan Eudiplodinium maggii to utilize chitin
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Acetylglucosamine metabolism MeSH
- Rumen parasitology MeSH
- Chitin isolation & purification metabolism MeSH
- Ciliophora isolation & purification metabolism MeSH
- Fungi chemistry MeSH
- Culture Media chemistry MeSH
- Butyric Acid metabolism MeSH
- Acetic Acid metabolism MeSH
- Fatty Acids, Volatile metabolism MeSH
- Sheep MeSH
- Propionates metabolism MeSH
- Trisaccharides metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Acetylglucosamine MeSH
- Chitin MeSH
- chitotriose MeSH Browser
- Culture Media MeSH
- Butyric Acid MeSH
- Acetic Acid MeSH
- Fatty Acids, Volatile MeSH
- Propionates MeSH
- propionic acid MeSH Browser
- Trisaccharides MeSH
The ability was determined of the rumen ciliate Eudiplodinium maggii to utilize chitin from fungal cell wall. Cultivation experiments shoved that the population concentration (number of ciliates in vitro) was positively correlated with chitin doses. Cell extract prepared from the bacteria-free ciliates degraded colloidal chitin releasing 2.0 micromol reducing sugar per mg protein per h. End products of this reaction were chitotriose and N-acetylglucosamine. Incubation of the bacteria-free ciliates with chitin resulted in an increase in the concentration of acetic, propionic and butyric acids in the incubation medium. The production rate of total volatile fatty acids (VFA) by ciliates incubated with and without chitin was 45.0 and 30.5 pmol VFA per protozoan, respectively, the molar proportion of particular acids remaining unchanged.
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Chitinolytic enzymes of the rumen ciliate Eudiplodinium maggii
