Chitinolytic enzymes of the rumen ciliate Eudiplodinium maggii
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
22528307
PubMed Central
PMC3389239
DOI
10.1007/s12223-012-0133-6
Knihovny.cz E-zdroje
- MeSH
- bachor metabolismus mikrobiologie MeSH
- chitin metabolismus MeSH
- Ciliophora chemie enzymologie izolace a purifikace metabolismus MeSH
- koncentrace vodíkových iontů MeSH
- ovce MeSH
- protozoální proteiny chemie metabolismus MeSH
- stabilita enzymů MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- chitin MeSH
- protozoální proteiny MeSH
The ability of rumen ciliates to digest chitin is clearly recognized. We investigated the chitinolytic system of the rumen ciliate Eudiplodinium maggii. The ciliates were grown in a selectively faunated sheep. They were isolated from the rumen and purified by sedimentation. A crude enzyme preparation was prepared following incubation of ciliates with antibiotics. This was done in order to reduce their contamination with intracellular bacteria. The activity of particular enzymes was examined by quantification of the products released from specific substrates. It was stated that the optimum conditions for the detected activities varied between 4.5 and 5.5 pH, and 45 and 55 °C. β-N-Acetylglucosaminidase was found as an enzyme of the highest activity (4.2 μmol/l released product per mg protein per h). The activities of endochitinase and exochitinase were almost two times lower than that of β-N-acetylglucosaminidase. Zymographic studies revealed the presence of two endochitinases, two exochitinases and two β-N-acetylglucosaminidases in the examined preparation.
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