Verification of reference genes for relative quantification of gene expression by real-time reverse transcription PCR in the pig
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18670063
DOI
10.1007/bf03195623
PII: 458
Knihovny.cz E-resources
- MeSH
- DNA-Binding Proteins genetics metabolism MeSH
- DNA Topoisomerases, Type II genetics metabolism MeSH
- Peptide Elongation Factor 1 genetics metabolism MeSH
- Gene Expression * MeSH
- Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) genetics metabolism MeSH
- Hypoxanthine Phosphoribosyltransferase genetics metabolism MeSH
- RNA, Messenger genetics metabolism MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Swine genetics MeSH
- Reference Standards MeSH
- Tissue Distribution MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA-Binding Proteins MeSH
- DNA Topoisomerases, Type II MeSH
- Peptide Elongation Factor 1 MeSH
- Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) MeSH
- Hypoxanthine Phosphoribosyltransferase MeSH
- RNA, Messenger MeSH
The aim of this study was to develop a set of reliable reference genes for quantification of mRNA expression in the pig. The mRNA expression stability in pig tissues was studied for 4 genes: EEF1A1, GAPDH, HPRT1 and TOP2B. The level of expression was characterized by Ct values for each gene and each tissue. By using the geNorm algorithm, the stability of the reference genes was determined in the diaphragm, heart, kidney, liver, lungs, longissimus muscle, and spleen. On the basis of this information, suitable reference genes can be selected for mRNA expression studies in relevant pig tissues.
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