Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- Cryptosporidium klasifikace genetika izolace a purifikace MeSH
- DNA fingerprinting MeSH
- feces parazitologie MeSH
- fylogeneze MeSH
- genotyp MeSH
- jatka MeSH
- kryptosporidióza parazitologie veterinární MeSH
- molekulární sekvence - údaje MeSH
- nemoci prasat parazitologie MeSH
- oocysty cytologie MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- prasata parazitologie MeSH
- protozoální DNA chemie genetika MeSH
- protozoální proteiny genetika MeSH
- ribozomální DNA chemie genetika MeSH
- RNA ribozomální 18S genetika MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- protozoální DNA MeSH
- protozoální proteiny MeSH
- ribozomální DNA MeSH
- RNA ribozomální 18S MeSH
A total of 123 fecal samples of slaughtered finisher pigs and 21 sows from 14 farms were screened for Cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method. Positive samples were molecularly characterized by direct sequencing of partial small subunit ribosomal RNA (SSU rRNA) and GP60 partial genes and polymerase chain reaction restriction fragment length polymorphism of SSU rRNA. Cryptosporidium oocysts were microscopically identified in 36 finishers (29%) and two sows (10%). Twenty-one mono-infections of Cryptosporidium pig genotype II and 15 mixed-infection of Cryptosporidium pig genotype II and Cryptosporidium suis in finishers were found. Both sows were infected with the Cryptosporidium parvum subgenotype IIaA16G1R1, which is reported from pigs for the first time.
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Aust Vet J. 1999 Jan;77(1):44-7 PubMed
Appl Environ Microbiol. 1997 Oct;63(10):3946-9 PubMed
Jpn J Infect Dis. 2001 Feb;54(1):23-6 PubMed
J Vet Med B Infect Dis Vet Public Health. 2001 Mar;48(2):151-9 PubMed
J Infect Dis. 2001 Feb 1;183(3):492-7 PubMed
Vet Parasitol. 1992 Jun;43(1-2):25-36 PubMed
Vet Parasitol. 1994 Apr;52(3-4):331-6 PubMed
Vet Parasitol. 2006 Oct 10;141(1-2):48-59 PubMed
J Clin Microbiol. 2003 Jun;41(6):2744-7 PubMed
J Vet Med B Infect Dis Vet Public Health. 2006 Jun;53(5):239-43 PubMed
Vet Parasitol. 2007 Sep 30;148(3-4):231-5 PubMed
Vet Parasitol. 2008 May 31;153(3-4):220-4 PubMed
Vet Rec. 1987 Jun 27;120(26):615-6 PubMed
Parasitology. 2007 Mar;134(Pt 3):351-8 PubMed
Vet Parasitol. 2003 Apr 2;113(1):7-18 PubMed
J Am Vet Med Assoc. 1987 Mar 15;190(6):695-8 PubMed
Appl Environ Microbiol. 2006 Jun;72(6):4461-3 PubMed
Appl Environ Microbiol. 2005 Aug;71(8):4446-54 PubMed
Vet Parasitol. 2007 May 31;146(3-4):357-62 PubMed
Parasitol Res. 2006 Sep;99(4):346-52 PubMed
Parasitology. 2007 Mar;134(Pt 3):339-50 PubMed
Folia Parasitol (Praha). 1985;32(1):50 PubMed
J Am Vet Med Assoc. 1977 Feb 1;170(3):348-50 PubMed
Vet Parasitol. 1982 Nov;11(2-3):121-6 PubMed
Vet Pathol. 1981 Mar;18(2):248-55 PubMed
Parasitology. 2007 Oct;134(Pt 11):1575-82 PubMed
Review of Cryptosporidium and Giardia in the eastern part of Europe, 2016
Cryptosporidium scrofarum n. sp. (Apicomplexa: Cryptosporidiidae) in domestic pigs (Sus scrofa)
GENBANK
EU647727, EU647728
