From 2011 to 2012, to identify Cryptosporidium spp. occurrence in Eurasian wild boars (Sus scrofa) 29 randomly selected localities (both forest areas and enclosures) across the Central European countries of Austria, the Czech Republic, Poland, and the Slovak Republic were investigated. Cryptosporidium oocysts were microscopicaly detected in 11 out of 460 faecal samples examined using aniline-carbol-methyl violet staining. Sixty-one Cryptosporidium infections, including the 11 infections that were detected by microscopy, were detected using genus- or species-specific nested PCR amplification of SSU rDNA. This represents a 5.5 fold greater sensitivity for PCR relative to microscopy. Combining genus- and species-specific PCR tools significantly changes the perspective on the occurrence of Cryptosporidium spp. in wild boars. While RFLP and direct sequencing of genus specific PCR-amplified products revealed 56 C. suis (20) and C. scrofarum (36) monoinfections and only 5 mixed infections of these species, species-specific molecular tools showed 44 monoinfections and 17 mixed infections with these species. PCR analysis of the gp60 gene did not reveal any other Cryptosporidium infections. Similar to domestic pigs, C. scrofarum was detected as a dominant species infecting adult Eurasian wild boars (Sus scrofa). Cryptosporidium infected wild boars did not show signs of clinical disease. This report is perhaps the most comprehensive survey of cryptosporidial infection in wild boars.

Faculty of Agriculture University of South Bohemia in České Budějovice Studentská 13 370 05 České Budějovice Czech Republic

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Alves M, Xiao L, Sulaiman I, Lal AA, Matos O, Antunes F. Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal. J. Clin. Microbiol. 2003;41:2744–2747. PubMed PMC

Atwill ER, Sweitzer RA, Pereira MGC, Gardner IA, Van Vuren D, Boyce WM. Prevalence of and associated risk factors for shedding Cryptosporidium parvum oocysts and Giardia cysts within feral pig populations in California. App. Environ. Microbiol. 1997;63:3946–3949. PubMed PMC

Bergeland ME. Necrotic enteritis in nursing piglets. Proc. Am. Assoc. Vet. Lab. Diagn. 1977;20:151–158.

Carval D, Ferriere R. A unified model for the coevolution of resistance, tolerance, and virulence. Evolution. 2010;64:2988–3009. PubMed

Castro-Hermida JA, Garcia-Presedo I, Gonzalez-Warleta M, Mezo M. Prevalence of Cryptosporidium and Giardia in roe deer (Capreolus capreolus) and wild boars (Sus scrofa) in Galicia (NW, Spain) Vet. Parasitol. 2011;179:216–219. PubMed

Chen F, Huang K. Prevalence and phylogenetic analysis of Cryptosporidium in pigs in eastern China. Zoonoses Publ. Health. 2007;54:393–400. PubMed

Fayer R. Taxonomy and species delimitation in Cryptosporidium. Exp. Parasitol. 2010;124:90–97. PubMed

Feng Y, Zhao X, Chen J, Jin W, Zhou X, Li N, Wang L, Xiao L. Occurrence, source, and human infection potential of Cryptosporidium and Giardia spp. in source and tap water in Shanghai, China. Appl. Environ. Microbiol. 2011;77:3609–3616. PubMed PMC

García-Presedo I, Pedraza-Díaz S, González-Warleta M, Mezo M, Gómez-Bautista M, Ortega-Mora LM, Castro-Hermida JA. Presence of Cryptosporidium scrofarum, C. suis and C. parvum subtypes IIaA16G2R1 and IIaA13G1R1 in Eurasian wild boars (Sus scrofa) Vet. Parasitol. 2013 doi:pii: S0304-4017(13)00229-X. PubMed

Guselle NJ, Appelbee AJ, Olson ME. Biology of Cryptosporidium parvum in pigs: from weaning to market. Vet. Parasitol. 2003;113:7–18. PubMed

Hamnes IS, Gjerde BK, Forberk T, Robertson LJ. Occurrence of Cryptosporidium and Giardia in suckling piglets in Norway. Vet. Parasitol. 2007;144:222–233. PubMed

Jeníková M, Němejc K, Sak B, Květoňová D, Kváč M. New view on the age-specificity of pig Cryptosporidium by species-specific primers for distinguishing Cryptosporidium suis and Cryptosporidium pig genotype II. Vet. Parasitol. 2011;176:120–125. PubMed

Jiang J, Alderisio KA, Xiao L. Distribution of Cryptosporidium genotypes in storm event water samples from three watersheds in New York. Appl. Environ. Microbiol. 2005;71:4446–4454. PubMed PMC

Johnson J, Buddle R, Reid S, Armson A, Ryan UM. Prevalence of Cryptosporidium genotypes in pre- and post-weaned pigs in Australia. Exp. Parasitol. 2008;119:418–421. PubMed

Kennedy GA, Kreitner GL, Strafuss AC. Cryptosporidiosis in three pigs. J. Am. Vet. Med. Assoc. 1977;170:348–350. PubMed

Kváč M, Hanzlíková D, Sak B, Květoňová D. Prevalence and age-related infection of Cryptosporidium suis, C. muris and Cryptosporidium pig genotype II in pigs on a farm complex in the Czech Republic. Vet. Parasitol. 2009a;160:319–322. PubMed

Kváč M, Sak B, Hanzlíková D, Kotilová J, Květoňová D. Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic. Parasitol. Res. 2009b;104:425–428. PubMed

Kváč M, Květoňová D, Sak B, Ditrich O. Cryptosporidium pig genotype II in immunocompetent man. Emerg. Infect. Dis. 2009c;15:982–983. PubMed PMC

Kváč M, Kestřánová M, Květoňová P, Kotková M, Ortega Y, McEvoy J, Sak B. Cryptosporidium tyzzeri and Cryptosporidium muris originated from wild West-European house mice (Mus musculus domesticus) and East-European house mice (Mus musculus musculus) are non-infectious for pigs. Exp. Parasitol. 2012;131:107–110. PubMed

Kváč M, Kestřánová M, Pinková M, Květoňová D, Kalinová J, Wagnerová P, Kotková M, Vítovec J, Ditrich O, McEvoy J, Stenger B, Sak B. Cryptosporidium scrofarum n. sp. (Apicomplexa: Cryptosporidiidae) in domestic pigs (Sus scrofa) Vet. Parasitol. 2013;191:218–227. PubMed PMC

Kváč M, Ondráčková Z, Květoňová D, Sak B, Vítovec J. Infectivity and pathogenicity of Cryptosporidium andersoni to a novel host southern multimammate mouse (Mastomys coucha) Vet. Parasitol. 2007;143:229–233. PubMed

Lambrechts L, Fellous S, Koella JC. Coevolutionary interactions between host and parasite genotypes. Trends Parasitol. 2006;22:12–16. PubMed

Langkjaer RB, Vigre H, Enemark HL, Maddox-Hyttel C. Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark. Parasitology. 2007;134:339–350. PubMed

Maddox-Hyttel C, Langkjaer RB, Enemark HL, Vigre H. Cryptosporidium and Giardia in different age groups of Danish cattle and pigs – occurrence and management associated risk factors. Vet. Parasitol. 2006;141:48–59. PubMed

Miláček P, Vítovec J. Differential staining of Cryptosporidia by aniline-carbolmethyl violet and tartrazine in smears from faeces and scrapings of intestinal mucosa. Folia Parasitol. 1985;32:50. PubMed

Morgan UM, Buddle R, Armson A, Elliot A, Thompson RCA. Molecular and biological characterisation of Cryptosporidium in pigs. Aust. Vet. J. 1999;77:44–47. PubMed

Němejc K, Sak B, Květoňová D, Hanzal V, Jeníková M, Kváč M. The first report on Cryptosporidium suis and Cryptosporidium pig genotype II in Eurasian wild boars (Sus scrofa) (Czech Republic) Vet. Parasitol. 2012;184:122–125. PubMed

Němejc K, Sak B, Květoňová D, Kernerová N, Rost M, Cama VA, Kváč M. Occurrence of Cryptosporidium suis and Cryptosporidium scrofarum on commercial swine farms in the Czech Republic and its associations with age and husbandry practices. Parasitol. Res. 2013;112:1143–1154. PubMed PMC

O'Donoghue PJ. Cryptosporidium and cryptosporidiosis in man and animals. Int. J. Parasitol. 1995;25:139–195. PubMed

Pereira M, das G, Atwill ER, Crawford MR, Lefebvre RB. DNA sequence similarity between California isolates of Cryptosporidium parvum. Appl. Environ. Microbiol. 1998;64:1584–1586. PubMed PMC

Quílez J, Sánchez-Acedo C, Clave A, del Cacho E, López-Bernad F. Prevalence of Cryptosporidium infections in pigs in Aragón (northeastern Spain) Vet. Parasitol. 1996;56:345–348. PubMed

Rašková V, Květoňová D, Sak B, McEvoy J, Edwinson A, Stenger B, Kváč M. Human cryptosporidiosis caused by Cryptosporidium tyzzeri and C. parvum isolates presumably transmitted from wild mice. J. Clin. Microbiol. 2013;51:360–362. PubMed PMC

Ryan UM, Samarasinghe B, Read C, Buddle JR, Robertson ID, Thompson RC. Identification of a novel Cryptosporidium genotype in pigs. Appl. Environ. Microbiol. 2003;69:3970–3974. PubMed PMC

Ryan UM, Monis P, Enemark HL, Sulaiman I, Samarasinghe B, Read C, Buddle R, Robertson I, Zhou L, Thompson RC, Xiao L. Cryptosporidium suis n. sp. (Apicomplexa: Cryptosporidiidae) in pigs (Sus scrofa) J. Parasitol. 2004;90:769–773. PubMed

Ryan UM, Read C, Hawkins P, Warnecke M, Swanson P, Griffith M, Deere D, Cunningham M, Cox P. Genotypes of Cryptosporidium from Sydney water catchment areas. Appl. Environ. Microbiol. 2005;98:1221–1229. PubMed

Sanford SE. Enteric cryptosporidial infection in pigs: 184 cases (1981–1985) J. Am. Vet. Med. Assoc. 1987;190:695–698. PubMed

Schley L, Roper TJ. Diet of wildboard Sus scrofa in Western Europe, with particular reference to consumption of agricultural crops. Mammal. Rew. 2003;33:43–56.

Suárez-Luengas L, Clavel A, Quílez J, Goñi-Cepero MP, Torres E, Sánchez-Acedo C, del Cacho E. Molecular characterization of Cryptosporidium isolates from pigs in Zaragoza (northeastern Spain) Vet. Parasitol. 2007;148:231–235. PubMed

Tanriverdi S, Arslan MO, Akiyoshi DE, Tzipori S, Widmer G. Identification of genotypically mixed Cryptosporidium parvum populations in humans and calves. Mol. Biochem. Parasitol. 2003;130:13–22. PubMed

Vítovec J, Hamadejová K, Landová L, Kváč M, Květoňová D, Sak B. Prevalence and pathogenicity of Cryptosporidium suis in pre- and post-weaned pigs. J. Vet. Med. B. 2006;53:239–243. PubMed

Waldron LS, Cheung-Kwok-Sang C, Power ML. Wildlife-associated Cryptosporidium fayeri in human, Australia. Emerg. Infect. Dis. 2010;16:2006–2007. PubMed PMC

Xiao L, Singh A, Limor J, Graczyk TK, Gradus S, Lal AA. Molecular characterization of Cryptosporidium oocysts in samples of raw surface water and wastewater. Appl. Environ. Microbiol. 2001;67:1097–1101. PubMed PMC

Xiao L, Bern C, Arrowood M, Sulaiman I, Zhou L, Kawai V, Vivar A, Lal AA, Gilman RH. Identification of the Cryptosporidium pig genotype in a human patient. J. Infect. Dis. 2002;185:1846–1848. PubMed

Xiao L, Fayer R, Ryan U, Upton SJ. Cryptosporidium taxonomy: recent advances and implications for public health. Clin. Micro. Rev. 2004;17:72–97. PubMed PMC

Xiao L, Moore JE, Ukoh U, Gatei W, Lowery CJ, Murphy TM, Dooley JS, Millar BC, Rooney PJ, Rao JR. Prevalence and identity of Cryptosporidium spp. in pig slurry. Appl. Environ. Microbiol. 2006;72:4461–4463. PubMed PMC

Xiao S, An W, Chen Z, Zhang D, Yu J, Yang M. Occurrences and genotypes of Cryptosporidium oocysts in river network of southern-eastern China. Parasitol. Res. 2012;110:1701–1709. PubMed

Zintl A, Neville D, Maguire D, Fanning S, Mulcahy G, Smith HV, De Waal T. Prevalence of Cryptosporidium species in intensively farmed pigs in Ireland. Parasitology. 2007;134:1575–1582. PubMed

Review of Cryptosporidium and Giardia in the eastern part of Europe, 2016