Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Cryptosporidium classification genetics isolation & purification MeSH
- DNA Fingerprinting MeSH
- Feces parasitology MeSH
- Phylogeny MeSH
- Genotype MeSH
- Abattoirs MeSH
- Cryptosporidiosis parasitology veterinary MeSH
- Molecular Sequence Data MeSH
- Swine Diseases parasitology MeSH
- Oocysts cytology MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Swine parasitology MeSH
- DNA, Protozoan chemistry genetics MeSH
- Protozoan Proteins genetics MeSH
- DNA, Ribosomal chemistry genetics MeSH
- RNA, Ribosomal, 18S genetics MeSH
- Sequence Analysis, DNA MeSH
- Cluster Analysis MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- DNA, Protozoan MeSH
- Protozoan Proteins MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 18S MeSH
A total of 123 fecal samples of slaughtered finisher pigs and 21 sows from 14 farms were screened for Cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method. Positive samples were molecularly characterized by direct sequencing of partial small subunit ribosomal RNA (SSU rRNA) and GP60 partial genes and polymerase chain reaction restriction fragment length polymorphism of SSU rRNA. Cryptosporidium oocysts were microscopically identified in 36 finishers (29%) and two sows (10%). Twenty-one mono-infections of Cryptosporidium pig genotype II and 15 mixed-infection of Cryptosporidium pig genotype II and Cryptosporidium suis in finishers were found. Both sows were infected with the Cryptosporidium parvum subgenotype IIaA16G1R1, which is reported from pigs for the first time.
See more in PubMed
Aust Vet J. 1999 Jan;77(1):44-7 PubMed
Appl Environ Microbiol. 1997 Oct;63(10):3946-9 PubMed
Jpn J Infect Dis. 2001 Feb;54(1):23-6 PubMed
J Vet Med B Infect Dis Vet Public Health. 2001 Mar;48(2):151-9 PubMed
J Infect Dis. 2001 Feb 1;183(3):492-7 PubMed
Vet Parasitol. 1992 Jun;43(1-2):25-36 PubMed
Vet Parasitol. 1994 Apr;52(3-4):331-6 PubMed
Vet Parasitol. 2006 Oct 10;141(1-2):48-59 PubMed
J Clin Microbiol. 2003 Jun;41(6):2744-7 PubMed
J Vet Med B Infect Dis Vet Public Health. 2006 Jun;53(5):239-43 PubMed
Vet Parasitol. 2007 Sep 30;148(3-4):231-5 PubMed
Vet Parasitol. 2008 May 31;153(3-4):220-4 PubMed
Vet Rec. 1987 Jun 27;120(26):615-6 PubMed
Parasitology. 2007 Mar;134(Pt 3):351-8 PubMed
Vet Parasitol. 2003 Apr 2;113(1):7-18 PubMed
J Am Vet Med Assoc. 1987 Mar 15;190(6):695-8 PubMed
Appl Environ Microbiol. 2006 Jun;72(6):4461-3 PubMed
Appl Environ Microbiol. 2005 Aug;71(8):4446-54 PubMed
Vet Parasitol. 2007 May 31;146(3-4):357-62 PubMed
Parasitol Res. 2006 Sep;99(4):346-52 PubMed
Parasitology. 2007 Mar;134(Pt 3):339-50 PubMed
Folia Parasitol (Praha). 1985;32(1):50 PubMed
J Am Vet Med Assoc. 1977 Feb 1;170(3):348-50 PubMed
Vet Parasitol. 1982 Nov;11(2-3):121-6 PubMed
Vet Pathol. 1981 Mar;18(2):248-55 PubMed
Parasitology. 2007 Oct;134(Pt 11):1575-82 PubMed
Review of Cryptosporidium and Giardia in the eastern part of Europe, 2016
Cryptosporidium scrofarum n. sp. (Apicomplexa: Cryptosporidiidae) in domestic pigs (Sus scrofa)
GENBANK
EU647727, EU647728
