We describe the expression and distribution patterns of nestin, desmin and vimentin in intact and regenerating muscle spindles of the rat hind limb skeletal muscles. Regeneration was induced by intramuscular isotransplantation of extensor digitorum longus (EDL) or soleus muscles from 15-day-old rats into the EDL muscle of adult female inbred Lewis rats. The host muscles with grafts were excised after 7-, 16-, 21- and 29-day survival and immunohistochemically stained. Nestin expression in intact spindles in host muscles was restricted to Schwann cells of sensory and motor nerves. In transplanted muscles, however, nestin expression was also found in regenerating "spindle fibers", 7 and 16 days after grafting. From the 21st day onwards, the regenerated spindle fibers were devoid of nestin immunoreactivity. Desmin was detected in spindle fibers at all developmental stages in regenerating as well as in intact spindles. Vimentin was expressed in cells of the outer and inner capsules of all muscle spindles and in newly formed myoblasts and myotubes of regenerating spindles 7 days after grafting. Our results show that the expression pattern of these intermediate filaments in regenerating spindle fibers corresponds to that found in regenerating extrafusal fibers, which supports our earlier suggestion that they resemble small-diameter extrafusal fibers.

Department of Histology and Embryology Faculty of Medicine in Hradec Kralove Charles University Prague Simkova 870 Hradec Králové 500 38 Czech Republic

Zobrazit více v PubMed

Proc Natl Acad Sci U S A. 1978 Oct;75(10):5034-8 PubMed

Exp Cell Res. 2007 Jun 10;313(10):2050-62 PubMed

Cell Mol Life Sci. 2004 Oct;61(19-20):2510-22 PubMed

J Oral Pathol Med. 2007 Sep;36(8):501-4 PubMed

Am J Surg Pathol. 1998 Nov;22(11):1368-78 PubMed

J Cell Biol. 1997 Oct 6;139(1):129-44 PubMed

APMIS. 2002 Jun;110(6):499-507 PubMed

Dev Biol. 1982 Dec;94(2):265-83 PubMed

Acta Neuropathol. 2001 Nov;102(5):473-84 PubMed

J Cell Sci. 1993 Dec;106 ( Pt 4):1291-300 PubMed

Stem Cells Dev. 2004 Dec;13(6):658-64 PubMed

Anat Embryol (Berl). 1995 Sep;192(3):283-91 PubMed

Cell. 1994 Nov 18;79(4):679-94 PubMed

Neuroscience. 1981;6(1):87-94 PubMed

Proc Natl Acad Sci U S A. 1981 Jan;78(1):298-302 PubMed

Exp Cell Res. 1999 Aug 25;251(1):213-23 PubMed

J Cell Biol. 1989 Aug;109(2):517-27 PubMed

Mech Dev. 1991 Aug;35(1):3-11 PubMed

Microsc Res Tech. 1995 Apr 1;30(5):390-407 PubMed

Cells Tissues Organs. 2009;189(5):338-47 PubMed

Am J Pathol. 1999 Feb;154(2):591-600 PubMed

J Biophys Biochem Cytol. 1960 Jul;7:725-42 PubMed

J Neurosci. 1985 Dec;5(12):3310-28 PubMed

Acta Neuropathol. 1995;89(1):85-95 PubMed

Neuroscience. 1993 Jan;52(1):207-18 PubMed

Histochemistry. 1976 Apr 21;47(1):43-57 PubMed

Neuromuscul Disord. 2004 Jul;14(7):421-8 PubMed

J Neurocytol. 1976 Aug;5(4):425-46 PubMed

Differentiation. 1997 Dec;62(4):179-86 PubMed

Histol Histopathol. 1989 Jan;4(1):47-53 PubMed

Acta Neuropathol. 2000 Oct;100(4):435-44 PubMed

Exp Cell Res. 1994 Oct;214(2):447-58 PubMed

J Neuropathol Exp Neurol. 2001 Jun;60(6):588-97 PubMed

Histol Histopathol. 2005 Apr;20(2):665-71 PubMed

Glia. 1988;1(2):105-12 PubMed

Cell. 1990 Feb 23;60(4):585-95 PubMed

J Comp Neurol. 1990 May 1;295(1):43-51 PubMed

J Neurochem. 1982 Mar;38(3):774-80 PubMed

J Histochem Cytochem. 1984 May;32(5):473-6 PubMed

Dev Biol. 1994 Sep;165(1):216-28 PubMed

J Embryol Exp Morphol. 1986 Mar;92:223-54 PubMed

J Neurosurg. 1988 Sep;69(3):429-35 PubMed

Lab Invest. 1987 Nov;57(5):480-8 PubMed

Pathol Int. 2007 Feb;57(2):60-7 PubMed

Nestin expression in intact and hypertrophic myocardium of spontaneously hypertensive rats during aging