Antibody response to the 45 kDa Candida albicans antigen in an animal model and potential role of the antigen in adherence
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Antigens, Fungal administration & dosage chemistry immunology MeSH
- Biofilms growth & development MeSH
- Cell Adhesion * MeSH
- Candida albicans growth & development immunology pathogenicity physiology MeSH
- Epithelial Cells microbiology MeSH
- Fungal Proteins administration & dosage chemistry immunology MeSH
- Immunization MeSH
- Rabbits MeSH
- Humans MeSH
- Disease Models, Animal * MeSH
- Molecular Sequence Data MeSH
- Antibodies, Fungal blood MeSH
- Receptors, Complement administration & dosage chemistry immunology MeSH
- Amino Acid Sequence MeSH
- Mouth Mucosa cytology microbiology MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, Fungal MeSH
- Fungal Proteins MeSH
- Antibodies, Fungal MeSH
- Receptors, Complement MeSH
The Candida antigen CR3-RP (complement receptor 3-related protein) is supposed to be a 'mimicry' protein because of its ability to bind antibody directed against the alpha subunit of the mammalian CR3 (CD11b/CD18). This study aimed to (i) investigate the specific humoral isotypic response to immunization with CR3-RP in vivo in a rabbit animal model, and (ii) determine the role of CR3-RP in the adherence of Candida albicans in vitro using the model systems of buccal epithelial cells (BECs) and biofilm formation. The synthetic C. albicans peptide DINGGGATLPQ corresponding to 11 amino-acids of the CR3-RP sequence DINGGGATLPQALXQITGVIT, determined by N-terminal sequencing, was used for immunization of rabbits to obtain polyclonal anti-CR3-PR serum and for subsequent characterization of the humoral isotypic response of rabbits. A significant increase of IgG, IgA and IgM anti-CR3-RP specific antibodies was observed after the third (P<0.01) and the fourth (P<0.001) immunization doses. The elevation of IgA levels suggested peptide immunomodulation of the IgA1 subclass, presumably in coincidence with Candida epithelial adherence. Blocking CR3-RP with polyclonal anti-CR3-RP serum reduced the ability of Candida to adhere to BECs, in comparison with the control, by up to 35 % (P<0.001), and reduced biofilm formation by 28 % (P<0.001), including changes in biofilm thickness and integrity detected by confocal laser scanning microscopy. These properties of CR3-RP suggest that it has potential for future vaccine development.
Department of Biophotonics International Laser Centre Bratislava Slovakia
Department of Hygiene Microbiology and Social Medicine Innsbruck Medical University Austria
Institute of Chemistry Slovak Academy of Sciences Bratislava Slovakia
Institute of Molecular Biology Slovak Academy of Sciences Bratislava Slovakia
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