Antibody response to the 45 kDa Candida albicans antigen in an animal model and potential role of the antigen in adherence
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- antigeny fungální aplikace a dávkování chemie imunologie MeSH
- biofilmy růst a vývoj MeSH
- buněčná adheze * MeSH
- Candida albicans růst a vývoj imunologie patogenita fyziologie MeSH
- epitelové buňky mikrobiologie MeSH
- fungální proteiny aplikace a dávkování chemie imunologie MeSH
- imunizace MeSH
- králíci MeSH
- lidé MeSH
- modely nemocí na zvířatech * MeSH
- molekulární sekvence - údaje MeSH
- protilátky fungální krev MeSH
- receptory komplementu aplikace a dávkování chemie imunologie MeSH
- sekvence aminokyselin MeSH
- ústní sliznice cytologie mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antigeny fungální MeSH
- fungální proteiny MeSH
- protilátky fungální MeSH
- receptory komplementu MeSH
The Candida antigen CR3-RP (complement receptor 3-related protein) is supposed to be a 'mimicry' protein because of its ability to bind antibody directed against the alpha subunit of the mammalian CR3 (CD11b/CD18). This study aimed to (i) investigate the specific humoral isotypic response to immunization with CR3-RP in vivo in a rabbit animal model, and (ii) determine the role of CR3-RP in the adherence of Candida albicans in vitro using the model systems of buccal epithelial cells (BECs) and biofilm formation. The synthetic C. albicans peptide DINGGGATLPQ corresponding to 11 amino-acids of the CR3-RP sequence DINGGGATLPQALXQITGVIT, determined by N-terminal sequencing, was used for immunization of rabbits to obtain polyclonal anti-CR3-PR serum and for subsequent characterization of the humoral isotypic response of rabbits. A significant increase of IgG, IgA and IgM anti-CR3-RP specific antibodies was observed after the third (P<0.01) and the fourth (P<0.001) immunization doses. The elevation of IgA levels suggested peptide immunomodulation of the IgA1 subclass, presumably in coincidence with Candida epithelial adherence. Blocking CR3-RP with polyclonal anti-CR3-RP serum reduced the ability of Candida to adhere to BECs, in comparison with the control, by up to 35 % (P<0.001), and reduced biofilm formation by 28 % (P<0.001), including changes in biofilm thickness and integrity detected by confocal laser scanning microscopy. These properties of CR3-RP suggest that it has potential for future vaccine development.
Department of Biophotonics International Laser Centre Bratislava Slovakia
Department of Hygiene Microbiology and Social Medicine Innsbruck Medical University Austria
Institute of Chemistry Slovak Academy of Sciences Bratislava Slovakia
Institute of Molecular Biology Slovak Academy of Sciences Bratislava Slovakia
Citace poskytuje Crossref.org
