Surface modification of hydrogels based on poly(2-hydroxyethyl methacrylate) with extracellular matrix proteins
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- extracelulární matrix - proteiny chemie MeSH
- hydrogely chemie MeSH
- koncentrace vodíkových iontů MeSH
- mikroskopie atomárních sil MeSH
- polyhydroxyethylmethakrylát analogy a deriváty chemie MeSH
- povrchové vlastnosti MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- extracelulární matrix - proteiny MeSH
- hydrogely MeSH
- polyhydroxyethyl methacrylate hydrogels MeSH Prohlížeč
- polyhydroxyethylmethakrylát MeSH
Infrared attenuated total reflection spectroscopy was used for in situ observation of the deposition of collagen I on poly(2-hydroxyethyl methacrylate-co-methacrylic acid, 2.9%) hydrogels and subsequent attachment of laminin or fibronectin on the collagen surface. While there was no adsorption of collagen dissolved in an acid solution on the hydrogel surface, it deposited on the surface at pH 6.5. The collagen layers with attached laminin or fibronectin were stable on hydrogel surface in physiological solution. The modification with collagen and particularly with collagen and laminin or fibronectin allowed the adhesion and growth of mesenchymal stromal cells and astrocytes on the hydrogel surface.
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