Effect of beta-hydroxy-beta-methylbutyrate (HMB) on protein metabolism in whole body and in selected tissues
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19056452
DOI
10.1016/j.fct.2008.11.021
PII: S0278-6915(08)00648-0
Knihovny.cz E-resources
- MeSH
- Liver drug effects metabolism MeSH
- Muscle, Skeletal drug effects metabolism MeSH
- Rats MeSH
- Kidney drug effects metabolism MeSH
- Myocardium metabolism MeSH
- Amino-Acid N-Acetyltransferase metabolism MeSH
- Rats, Wistar MeSH
- Proteins metabolism MeSH
- Spleen drug effects metabolism MeSH
- Heart drug effects MeSH
- Intestines drug effects MeSH
- Intestinal Mucosa metabolism MeSH
- Valerates pharmacology MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- beta-hydroxyisovaleric acid MeSH Browser
- Amino-Acid N-Acetyltransferase MeSH
- Proteins MeSH
- Valerates MeSH
Beta-hydroxy-beta-methylbutyrate (HMB) is a leucine metabolite with protein anabolic effect. The aim of the study was to examine the role of exogenous HMB on leucine and protein metabolism in whole body and selected tissues. Rats were administered by HMB (0.1 g/kg b.w.) or by saline. The parameters of whole-body protein metabolism were evaluated 24 h later using L-[1-14C]leucine and L-[3,4,5-3H]phenylalanine. Changes in proteasome dependent proteolysis and protein synthesis were determined according the "chymotrypsin-like" enzyme activity and labeled leucine and phenylalanine incorporation into the protein. A decrease in leucine clearance and whole-body protein turnover (i.e., a decrease in whole-body proteolysis and protein synthesis) was observed in HMB treated rats. Proteasome-dependent proteolysis decreased significantly in skeletal muscle, changes in heart, liver, jejunum, colon, kidney, and spleen were insignificant. Decrease in protein synthesis was observed in the heart, colon, kidney, and spleen, while an increase was observed in the liver. There were no significant changes in leucine oxidation. We conclude that protein anabolic effect of HMB in skeletal muscle is related to inhibition of proteolysis in proteasome. Alterations in protein synthesis in visceral tissues may affect several important functions and the metabolic status of the whole body.
References provided by Crossref.org
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