Differentiation of Plum pox virus isolates by single-strand conformation polymorphism and low-stringency single specific primer PCR analysis of HC-Pro genome region
Jazyk angličtina Země Švýcarsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
19301952
Knihovny.cz E-zdroje
- MeSH
- cysteinové endopeptidasy genetika MeSH
- polymerázová řetězová reakce metody MeSH
- polymorfismus genetický * MeSH
- polymorfismus konformace jednovláknové DNA MeSH
- RNA virová genetika MeSH
- virové proteiny genetika MeSH
- virus šarky švestky klasifikace genetika izolace a purifikace MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- cysteinové endopeptidasy MeSH
- HC-Pro protein, potyvirus MeSH Prohlížeč
- RNA virová MeSH
- virové proteiny MeSH
Single-strand conformation polymorphism (SSCP) and low-stringency single specific primer (LSSP)-PCR were assessed for suitability and reliability in genotyping of Plum pox virus (PPV) isolates. Examined PPV isolates included 16 PPV-D, 12 PPV-M, and 14 PPV-Rec isolates collected in Czech Republic. The analysis was performed on the helper component protease (HC-Pro) region of the PPV genome. SSCP and LSSP-PCR allowed the differentiation of PPV strain, but SSCP was not able to distinguish isolates within the same strain. The individual genotyping of each PPV isolate was obtained by LSSP-PCR. Nevertheless, both SSCP and LSSP-PCR techniques are suitable for preliminary screening of genetic variability of plant RNA viruses.
