Differentiation of Plum pox virus isolates by single-strand conformation polymorphism and low-stringency single specific primer PCR analysis of HC-Pro genome region
Language English Country Switzerland Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19301952
Knihovny.cz E-resources
- MeSH
- Cysteine Endopeptidases genetics MeSH
- Polymerase Chain Reaction methods MeSH
- Polymorphism, Genetic * MeSH
- Polymorphism, Single-Stranded Conformational MeSH
- RNA, Viral genetics MeSH
- Viral Proteins genetics MeSH
- Plum Pox Virus classification genetics isolation & purification MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- Cysteine Endopeptidases MeSH
- HC-Pro protein, potyvirus MeSH Browser
- RNA, Viral MeSH
- Viral Proteins MeSH
Single-strand conformation polymorphism (SSCP) and low-stringency single specific primer (LSSP)-PCR were assessed for suitability and reliability in genotyping of Plum pox virus (PPV) isolates. Examined PPV isolates included 16 PPV-D, 12 PPV-M, and 14 PPV-Rec isolates collected in Czech Republic. The analysis was performed on the helper component protease (HC-Pro) region of the PPV genome. SSCP and LSSP-PCR allowed the differentiation of PPV strain, but SSCP was not able to distinguish isolates within the same strain. The individual genotyping of each PPV isolate was obtained by LSSP-PCR. Nevertheless, both SSCP and LSSP-PCR techniques are suitable for preliminary screening of genetic variability of plant RNA viruses.
