Yeast vitality determination based on intracellular NAD(P)H fluorescence measurement during aerobic-anaerobic transition
Language English Country United States Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Aerobiosis MeSH
- Anaerobiosis MeSH
- Spectrometry, Fluorescence methods MeSH
- Microbial Viability * MeSH
- NAD metabolism MeSH
- NADP metabolism MeSH
- Saccharomyces cerevisiae growth & development metabolism MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- NAD MeSH
- NADP MeSH
This work presents a yeast-cell vitality-assessment method based on on-line intracellular fluorescence measurement. The intracellular NAD(P)H fluorescence of a cell suspension is recorded during transition from aerobic to anaerobic conditions and the output signal is evaluated as a measure of yeast vitality (quality). This fluorescence method showed a highly satisfactory correlation with even low dead cell numbers where the acidification power test could not be applied.
See more in PubMed
Folia Microbiol (Praha). 2008;53(5):451-6 PubMed
Biotechnol Prog. 1998 Jan-Feb;14(1):63-74 PubMed
Biotechnol Bioeng. 1991 May;37(11):1043-9 PubMed
Folia Microbiol (Praha). 2003;48(2):189-92 PubMed
Biotechnol Prog. 2005 May-Jun;21(3):653-63 PubMed
Folia Microbiol (Praha). 1982;27(6):395-403 PubMed
Folia Microbiol (Praha). 2006;51(6):525-34 PubMed
Biotechnol Prog. 2004 Nov-Dec;20(6):1733-40 PubMed
