S. cerevisiae growth and responses to different treatments were monitored by two-dimensional fluorescence spectroscopy, which simultaneously detects the fluorescence of a number of cells' own fluorophores. Growth curves of cultures of free cells were measured by means of tryptophan fluorescence in nonfluorescent culture medium and a flow-through system at a suitable excitation/emission beam geometry. Fast responses of the cells to anaerobic-aerobic transition or addition of glucose, methanol or cyanide, which could not be measured in this system because of the time delay inherent in transporting the cells from the culture flask to the cuvette, were monitored with cells immobilized in alginate. The major fluorescence changes caused by these treatments belonged to NAD(P)H which is a good indicator of the redox state of the cells.

Institute of Chemical Process Fundamentals Academy of Sciences of the Czech Republic 165 02 Prague 6 Czechia

Folia Microbiol (Praha). 2003;48(5):704 PubMed

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Biochem Biophys Res Commun. 1978 Aug 14;83(3):1225-33 PubMed

Biotechnol Prog. 1991 Jan-Feb;7(1):21-7 PubMed

J Biotechnol. 2001 Jun 1;88(1):47-57 PubMed

Biotechnol Prog. 1998 Jan-Feb;14(1):63-74 PubMed

Biotechnol Prog. 1996 Jan-Feb;12(1):126-31 PubMed

Biotechnol Bioeng. 1989 Aug 20;34(5):660-70 PubMed

Biotechnol Bioeng. 1991 Jan 20;37(2):141-59 PubMed

Biotechnol Prog. 1993 Nov-Dec;9(6):666-70 PubMed

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Yeast vitality determination based on intracellular NAD(P)H fluorescence measurement during aerobic-anaerobic transition