Detection of antibodies to Chlamydophila pneumoniae by immunoblotting in patients with cardiovascular diseases
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
19358448
Knihovny.cz E-zdroje
- MeSH
- Chlamydophila pneumoniae imunologie izolace a purifikace MeSH
- imunoblotting MeSH
- imunoglobuliny krev MeSH
- kardiovaskulární nemoci mikrobiologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- protilátky bakteriální krev MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- imunoglobuliny MeSH
- protilátky bakteriální MeSH
Chlamydophila pneumoniae, one of the most prevalent human pathogens worldwide, is not only a significant cause of pneumonia, but may also be associated with cardiovascular diseases (CVD) as suggested by multiple studies. A total of 228 sera from CVD patients with hypertension, ischemic heart disease or previous reconstructive vascular surgery were screened for the presence of anti-C. pneumoniae IgG and IgA antibodies by ELISA. Out of 150 positive serum samples, 80 with similar IgG and IgA levels were investigated by immunoblot (IB). IgG antibodies were directed predominantly against the 35 kDa and 39 kDa proteins as well as 50-54 and 56-60 kDa proteins of C. pneumoniae. IgA antibodies reacted most frequently with the 50-54 and 56-60 kDa proteins.
