Cyclin T2 is essential for mouse embryogenesis
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
Grant support
P50 GM082250
NIGMS NIH HHS - United States
PubMed
19364821
PubMed Central
PMC2698739
DOI
10.1128/mcb.00172-09
PII: MCB.00172-09
Knihovny.cz E-resources
- MeSH
- Cyclin T antagonists & inhibitors deficiency genetics metabolism MeSH
- Cyclins antagonists & inhibitors deficiency genetics metabolism MeSH
- DNA Primers genetics MeSH
- Embryonic Stem Cells metabolism MeSH
- Embryonic Development genetics physiology MeSH
- Genes, Lethal MeSH
- RNA, Small Interfering genetics MeSH
- Mice, Mutant Strains MeSH
- Mice, Knockout MeSH
- Mice MeSH
- Positive Transcriptional Elongation Factor B metabolism MeSH
- Base Sequence MeSH
- Pregnancy MeSH
- Tissue Distribution MeSH
- Gene Expression Regulation, Developmental MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Pregnancy MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, N.I.H., Extramural MeSH
- Names of Substances
- Ccnt1 protein, mouse MeSH Browser
- Cyclin T MeSH
- Cyclins MeSH
- DNA Primers MeSH
- RNA, Small Interfering MeSH
- Positive Transcriptional Elongation Factor B MeSH
The positive transcription elongation factor b (P-TEFb) is essential for the elongation of transcription and cotranscriptional processing by RNA polymerase II. In mammals, it contains predominantly the C-type cyclin cyclin T1 (CycT1) or CycT2 and cyclin-dependent kinase 9 (Cdk9). To determine if these cyclins have redundant functions or affect distinct sets of genes, we genetically inactivated the CycT2 gene (Ccnt2) using the beta-galactosidase-neomycin gene (beta-geo) gene trap technology in the mouse. Visualizing beta-galactosidase during mouse embryogenesis revealed that CycT2 is expressed abundantly during embryogenesis and throughout the organism in the adult. This finding was reflected in the expression of CycT2 in all adult tissues and organs. However, despite numerous matings of heterozygous mice, we observed no CycT2(-/-) embryos, pups, or adult mice. This early lethality could have resulted from decreased expression of critical genes, which were revealed by short interfering RNAs against CycT2 in embryonic stem cells. Thus, CycT1 and CycT2 are not redundant, and these different P-TEFb complexes regulate subsets of distinct genes that are important for embryonic development.
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