Staphylococcus cohnii hemolysins - isolation, purification and properties
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
- MeSH
- dospělí MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- hemolyziny izolace a purifikace metabolismus MeSH
- infekce spojené se zdravotní péčí mikrobiologie MeSH
- isoelektrická fokusace MeSH
- kůže mikrobiologie MeSH
- lidé MeSH
- molekulová hmotnost MeSH
- novorozenec MeSH
- personál nemocniční MeSH
- stafylokokové infekce mikrobiologie MeSH
- Staphylococcus chemie izolace a purifikace MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- novorozenec MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Geografické názvy
- Polsko MeSH
- Názvy látek
- hemolyziny MeSH
A total 355 of Staphylococcus cohnii isolates from hospital environment, patients (newborns), medical staff and from non-hospital environment were tested for hemolytic activity. Ninety-one % of S. cohnii ssp. cohnii and 74.5 % S. cohnii ssp. urealyticus strains exhibited hemolysis synergistic to S. aureus ATCC 25923 strain. Crude preparations of hemolysins of both bacterial subspecies presented delta-hemolysin, but not alpha- and beta-toxin activity. Highly pure hemolysins were obtained by semipreparative SDS-PAGE or by organic solvent extraction from the freeze-dried crude preparations. Native-PAGE and 2D-PAGE showed their high heterogeneity. Molar masses of single hemolysin units estimated by the Tris-Tricine-SDS-PAGE were calculated as 3.47 kDa for S. cohnii ssp. cohnii and 3.53 kDa for S. cohnii ssp. urealyticus.
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