Staphylococcus cohnii hemolysins - isolation, purification and properties
Language English Country United States Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Adult MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Hemolysin Proteins isolation & purification metabolism MeSH
- Cross Infection microbiology MeSH
- Isoelectric Focusing MeSH
- Skin microbiology MeSH
- Humans MeSH
- Molecular Weight MeSH
- Infant, Newborn MeSH
- Personnel, Hospital MeSH
- Staphylococcal Infections microbiology MeSH
- Staphylococcus chemistry isolation & purification MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Infant, Newborn MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Geographicals
- Poland MeSH
- Names of Substances
- Hemolysin Proteins MeSH
A total 355 of Staphylococcus cohnii isolates from hospital environment, patients (newborns), medical staff and from non-hospital environment were tested for hemolytic activity. Ninety-one % of S. cohnii ssp. cohnii and 74.5 % S. cohnii ssp. urealyticus strains exhibited hemolysis synergistic to S. aureus ATCC 25923 strain. Crude preparations of hemolysins of both bacterial subspecies presented delta-hemolysin, but not alpha- and beta-toxin activity. Highly pure hemolysins were obtained by semipreparative SDS-PAGE or by organic solvent extraction from the freeze-dried crude preparations. Native-PAGE and 2D-PAGE showed their high heterogeneity. Molar masses of single hemolysin units estimated by the Tris-Tricine-SDS-PAGE were calculated as 3.47 kDa for S. cohnii ssp. cohnii and 3.53 kDa for S. cohnii ssp. urealyticus.
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