Due to high resistance, standard chemotherapy of biofilm-associated staphylococcal infections is ineffective and a number of alternative approaches to antimicrobial treatment have been proposed. Minimum inhibitory concentration (MIC) and biofilm inhibitory concentration (BIC) of oxacillin (Oxa), vancomycin (Van), linezolid (Lzd) and lysostaphin (Lss) as well as the possible synergistic effect of the antibiotics and lysostaphin were determined. The Lss susceptibility of Staphylococcus aureus planktonic and bio-film cultures varied and was strain-dependent. The synergistic effect of sub-BIC(Lss)+Oxa was observed for methicillin-sensitive S. aureus (MSSa) and methicillin-resistant S. aureus (MrSa), but not for heterogeneously vancomycin-resistant S. aureus (V(h)Sa) biofilm. Van with sub-BICL(Lss) was effective against M(s)Sa and MrSa biofilm, when applied in three subsequent doses. Only sub-BICL(Lss)+Lzd combination, given as three cycles therapy, was effective in disruption of all 3 (M(s)Sa, M(r)Sa, V(h)Sa) biofilms.

Department of Immunology and Infectious Biology Institute of Microbiology and Immunology University of Lódz Poland

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J Antimicrob Chemother. 2001 Nov;48(5):617-25 PubMed

Pol J Microbiol. 2005;54(3):191-200 PubMed

Folia Microbiol (Praha). 2004;49(1):75-8 PubMed

Mol Microbiol. 2002 Mar;43(6):1367-78 PubMed

Antimicrob Agents Chemother. 2002 Feb;46(2):598-9 PubMed

Antimicrob Agents Chemother. 2001 Apr;45(4):999-1007 PubMed

Folia Microbiol (Praha). 2004;49(5):596-600 PubMed

Antimicrob Agents Chemother. 2001 May;45(5):1431-7 PubMed

J Antimicrob Chemother. 2003 May;51 Suppl 2:ii27-35 PubMed

J Microbiol Methods. 2003 Aug;54(2):269-76 PubMed

Antimicrob Agents Chemother. 2003 Nov;47(11):3407-14 PubMed

Vaccine. 1999 May 14;17 (19):2423-8 PubMed

J Antimicrob Chemother. 2002 Feb;49(2):301-8 PubMed

Antimicrob Agents Chemother. 2002 Jun;46(6):2017-20 PubMed

Nat Rev Microbiol. 2004 Feb;2(2):95-108 PubMed

Expert Rev Anti Infect Ther. 2003 Dec;1(4):667-83 PubMed

Invest Ophthalmol Vis Sci. 2002 Dec;43(12):3712-6 PubMed

Ann Clin Microbiol Antimicrob. 2005 Jan 07;4:2 PubMed

Antimicrob Agents Chemother. 2004 May;48(5):1681-7 PubMed

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