Biomarkers of exposure to tobacco smoke and environmental pollutants in mothers and their transplacental transfer to the foetus. Part II. Oxidative damage
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19433097
DOI
10.1016/j.mrfmmm.2009.04.010
PII: S0027-5107(09)00148-1
Knihovny.cz E-resources
- MeSH
- 8-Hydroxy-2'-Deoxyguanosine MeSH
- DNA Adducts blood MeSH
- Biomarkers blood MeSH
- Deoxyguanosine analogs & derivatives metabolism MeSH
- Adult MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- F2-Isoprostanes metabolism MeSH
- Fetal Blood chemistry MeSH
- Protein Carbonylation MeSH
- Cotinine analysis MeSH
- Smoking * MeSH
- Blood Proteins analysis MeSH
- Air Pollutants blood MeSH
- Humans MeSH
- Lymphocytes drug effects MeSH
- Maternal-Fetal Exchange MeSH
- Maternal Exposure * MeSH
- Young Adult MeSH
- Infant, Newborn MeSH
- Oxidation-Reduction MeSH
- Oxidative Stress * MeSH
- Lipid Peroxidation MeSH
- Placenta metabolism MeSH
- Polycyclic Aromatic Hydrocarbons blood MeSH
- Pregnancy MeSH
- Vitamin A analysis MeSH
- Vitamin E analysis MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Young Adult MeSH
- Infant, Newborn MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 5-F(2t)-isoprostane MeSH Browser
- 8-Hydroxy-2'-Deoxyguanosine MeSH
- DNA Adducts MeSH
- Biomarkers MeSH
- Deoxyguanosine MeSH
- F2-Isoprostanes MeSH
- Cotinine MeSH
- Blood Proteins MeSH
- Air Pollutants MeSH
- polycyclic aromatic hydrocarbons-DNA adduct MeSH Browser
- Polycyclic Aromatic Hydrocarbons MeSH
- Vitamin A MeSH
- Vitamin E MeSH
Oxidative damage to macromolecules may have numerous negative health consequences. We measured oxidative damage to DNA, proteins and lipids in 80 newborns and 79 mothers, analyzed the effect of mother's tobacco smoke exposure on oxidative stress, and assessed correlations between oxidative stress markers and bulky and PAH (polycyclic aromatic hydrocarbons)-specific DNA adducts. Mean levels (+/-S.D.) of 8-oxodeoxyguanosine (8-oxodG) per 10(5) dG in the placenta were 2.85+/-0.78; we did not see a difference between 8-oxodG levels in newborns born to mothers exposed and unexposed to tobacco smoke. Protein carbonyl levels, a marker of protein oxidation, were comparable in the umbilical cord and in maternal venous blood plasma (17.4+/-3.2 and 17.6+/-4.2nmol/ml plasma in newborns and mothers, respectively, p=0.66). Lipid peroxidation measured as levels of 15-F(2t)-isoprostane (15-F(2t)-IsoP) in plasma was significantly higher in newborns than in mothers (362+/-129 and 252+/-130pg/ml in newborns and mothers, respectively, p<0.001). We did not find any effect of tobacco smoke exposure on either biomarker in any group. Levels of both protein carbonyls and 15-F(2t)-IsoP in cord blood significantly correlated with those in maternal plasma (p<0.001). 8-oxodG levels positively correlated with plasma carbonyls in cord plasma, as well as with cotinine levels (marker of tobacco smoke exposure) in maternal plasma. 8-oxodG levels also correlated with bulky DNA adducts in lymphocyte DNA of newborns and mothers and with PAH-DNA adducts in the placenta. Our results showed higher lipid peroxidation in newborns than in mothers, close correlation of analyzed oxidative stress markers between newborns and mothers, and a relationship between oxidative stress and induction of DNA adducts.
Laboratory of Genetic Ecotoxicology Institute of Experimental Medicine AS CR Prague 4 Czech Republic
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