Spatial and temporal regulation of Wnt/beta-catenin signaling is essential for development of the retinal pigment epithelium
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
19596317
DOI
10.1016/j.ydbio.2009.07.002
PII: S0012-1606(09)01035-5
Knihovny.cz E-zdroje
- MeSH
- beta-katenin metabolismus MeSH
- buněčná diferenciace MeSH
- chromatinová imunoprecipitace MeSH
- embryo savčí metabolismus MeSH
- exprese genu MeSH
- imunohistochemie MeSH
- integrasy MeSH
- myši MeSH
- proteiny Wnt metabolismus MeSH
- retinální pigmentový epitel růst a vývoj metabolismus MeSH
- signální transdukce * MeSH
- transfekce MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- beta-katenin MeSH
- Cre recombinase MeSH Prohlížeč
- integrasy MeSH
- proteiny Wnt MeSH
Wnt/beta-catenin signaling is highly active in the dorsal retinal pigment epithelium (RPE) during eye development. To study the role of Wnt/beta-catenin signaling in the RPE development we used a conditional Cre/loxP system in mice to inactivate or ectopically activate Wnt/beta-catenin signaling in the RPE. Inactivation of Wnt/beta-catenin signaling results in transdifferentiation of RPE to neural retina (NR) as documented by downregulation of RPE-specific markers Mitf and Otx2 and ectopic expression of NR-specific markers Chx10 and Rx, respectively. In contrast, ectopic activation of Wnt/beta-catenin signaling results in the disruption of the RPE patterning, indicating that precise spatial and temporal regulation of Wnt/beta-catenin signaling is required for normal RPE development. Using chromatin immunoprecipitation (ChIP) and reporter gene assays we provide evidence that Otx2 and RPE-specific isoform of Mitf, Mitf-H, are direct transcriptional targets of Wnt/beta-catenin signaling. Combined, our data suggest that Wnt/beta-catenin signaling plays an essential role in development of RPE by maintaining or inducing expression of Mitf and Otx2.
Citace poskytuje Crossref.org
WNT/β-Catenin Signaling in Vertebrate Eye Development
Genetic interaction between Pax6 and β-catenin in the developing retinal pigment epithelium
