Direct ESI-MS analysis of O-acyl glycosylated cardiolipins from the thermophilic bacterium Alicyclobacillus acidoterrestris
Language English Country Ireland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19651116
DOI
10.1016/j.chemphyslip.2009.07.005
PII: S0009-3084(09)00294-1
Knihovny.cz E-resources
- MeSH
- Bacteria chemistry MeSH
- Glycosylation MeSH
- Spectrometry, Mass, Electrospray Ionization methods MeSH
- Mass Spectrometry methods MeSH
- Oxygen Isotopes chemistry MeSH
- Cardiolipins analysis isolation & purification MeSH
- Clinical Laboratory Techniques MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Oxygen Isotopes MeSH
- Cardiolipins MeSH
We used direct ESI-MS analysis to identify derivatives of cardiolipin molecular species (i.e. O-acyl glycosylated cardiolipins) from the thermophilic bacterium Alicyclobacillus acidoterrestris. We used triple-quadrupole type mass spectrometer for analysis of this complex lipid and enzymatic hydrolysis and 1H and 13C NMR for the identification of these cardiolipin derivatives. These techniques enabled us to identify and quantify the specific molecular species profiles of derivatives of cardiolipin directly from lipid extracts of the bacterium including the identification of the sugar moiety as alpha-D-mannose and all five acyls including their positional isomers.
References provided by Crossref.org
