Bifidobacteria in the digestive tract of bumblebees
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19651224
DOI
10.1016/j.anaerobe.2009.07.007
PII: S1075-9964(09)00115-2
Knihovny.cz E-resources
- MeSH
- Actinobacteria classification isolation & purification MeSH
- Aldehyde-Lyases metabolism MeSH
- Bacterial Proteins metabolism MeSH
- Bacteriological Techniques methods MeSH
- Nucleic Acid Denaturation MeSH
- DNA, Bacterial genetics MeSH
- DNA Fingerprinting MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Phylogeny MeSH
- Gastrointestinal Tract microbiology MeSH
- Culture Media chemistry MeSH
- Colony Count, Microbial MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Cluster Analysis MeSH
- Bacterial Typing Techniques MeSH
- Bees microbiology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Aldehyde-Lyases MeSH
- Bacterial Proteins MeSH
- DNA, Bacterial MeSH
- fructose-6-phosphate phosphoketolase MeSH Browser
- Culture Media MeSH
- RNA, Ribosomal, 16S MeSH
Bifidobacteria and other bacterial groups (lactobacilli, facultative anaerobes, anaerobes) from the digestive tract of three bumblebee species (Bombus lucorum (34 samples), Bombus pascuorum (18 samples) and Bombus lapidarius (9 samples)) were enumerated and characterised. Counts of facultative anaerobic bacteria and lactobacilli (5.41+/-2.92 and 2.69+/-3.02 log CFU/g of digestive tract content) were lower than those of anaerobes (7.66+/-0.86 log CFU/g). Counts of bifidobacteria were determined using two selective media: MTPY (Modified Trypticase Phytone Yeast extract agar) and a new medium with pollen extract. There was no significant difference between the counts of bifidobacteria from both media, 5.00+/-2.92 log CFU/g on MTPY and 5.00+/-2.87 on the pollen medium. Subsequently, 187 bacterial strains of the family Bifidobacteriaceae (fructose-6-phosphate phosphoketolase-positive) were isolated from three different localities and from all three species of bumblebees. Bifidobacteria were found in 42 out of 61 specimens (69%). Twenty-three (38%) specimens had counts of bifidobacteria higher than 7.0 log CFU/g. Bifidobacteria represented the dominant group of anaerobes (>70% of total anaerobes), i.e., the principal group of bacteria in the bumblebee digestive tract, in only fourteen specimens (23% of total). For the first time, bifidobacteria were isolated from the digestive tract of bumblebees. In addition, we suggest, on the basis of biochemical tests (API 50 CHL and RAPID ID 32) and genetic methods (PCR and DGGE), that these bacteria may represent new species within the family of Bifidobacteriaceae.
References provided by Crossref.org
Survival of bifidobacteria administered to calves
The presence of bifidobacteria in social insects, fish and reptiles
