Study of metallothionein oxidation by using of chip CE
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Analysis of Variance MeSH
- Electrophoresis, Capillary methods MeSH
- Metallothionein chemistry MeSH
- Microfluidic Analytical Techniques methods MeSH
- Oxidation-Reduction MeSH
- Hydrogen Peroxide chemistry MeSH
- Polymers chemistry MeSH
- Sensitivity and Specificity MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Metallothionein MeSH
- Hydrogen Peroxide MeSH
- Polymers MeSH
Metallothionein (MT) is a low-molecular mass protein playing an essential role in homeostasis of heavy metals ions. We used a chip-based CE for quantitative study of MT oxidation. After oxidation of MT by H(2)O(2) we observed marked decrease in peaks heights and shift of peaks positions to higher molecular mass, which corresponded with the time of the oxidation. Moreover, we observed that the proportion of high-molecular forms of MT was markedly increased. The oxidative changes were successfully reversed by using of reducing agent prior to electrophoresis. Our method can reveal the stability MT aggregates, of which biological role still remains unclear.
References provided by Crossref.org
Fluorescence-tagged metallothionein with CdTe quantum dots analyzed by the chip-CE technique
