Biodiversity of streptomycetes isolated from a succession sequence at a post-mining site and their evidence in Miocene lacustrine sediment
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20015625
DOI
10.1016/j.micres.2009.10.002
PII: S0944-5013(09)00100-1
Knihovny.cz E-resources
- MeSH
- Anti-Bacterial Agents metabolism MeSH
- Biodiversity * MeSH
- DNA, Bacterial genetics MeSH
- Phylogeny MeSH
- Geologic Sediments microbiology MeSH
- Mining MeSH
- Molecular Sequence Data MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Streptomyces classification genetics isolation & purification metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- DNA, Bacterial MeSH
- RNA, Ribosomal, 16S MeSH
The genetic diversity of streptomycetes in colliery spoil heaps (Sokolov, Czech Republic) was investigated by restriction pattern analysis of 16S-internal transcribed spacer rDNA and 16S sequences. We sampled freshly excavated Miocene sediment (17-19-million-year-old) and four sites of primary succession (initial, early, middle, and late stages; aged 1-44 years) on the same sediment. Active bacteria were present even in fresh Miocene sediment, and the relative proportion of actinomycetes among total bacterial and their genetic diversity increased significantly with the age of the sampling site. The replacement of pioneer species by late succession species during succession was observed. Plate assays of Streptomyces strains revealed 27% antibiotic-producing strains. Screening for nonribosomal peptide synthases and type I polyketide synthases systems suggested that 90% and 55% streptomycetes, respectively, are putative producers of biologically active compounds. The frequencies of tetracycline-, amoxicillin-, and chloramphenicol-resistant streptomycetes were 6%, 9%, and 15%, respectively. These findings document the occurrence of genetic elements encoding antibiotic resistance genes and the production of antibiotics by streptomycetes located in pristine environments. Our results indicate key roles for ancient streptomycetes related to S. microflavus, S. spororaveus, and S. flavofuscus in pioneering community development in freshly excavated substrates.
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EU098010, EU098011, EU098012, EU098013, EU098014, EU098015, EU098016, EU098017, EU098018, EU098019, EU098020, EU098021, EU098022, EU098023, EU098024, EU098025, EU098026, EU098027, EU098028, EU098029, EU098030, EU098031, EU098032, EU098033, EU098034, EU098035, EU098036, EU098037, EU098038, EU098039, EU098040, EU098041, EU098042, EU098043, EU098044, EU098045
