Biodiversity of streptomycetes isolated from a succession sequence at a post-mining site and their evidence in Miocene lacustrine sediment
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
20015625
DOI
10.1016/j.micres.2009.10.002
PII: S0944-5013(09)00100-1
Knihovny.cz E-zdroje
- MeSH
- antibakteriální látky metabolismus MeSH
- biodiverzita * MeSH
- DNA bakterií genetika MeSH
- fylogeneze MeSH
- geologické sedimenty mikrobiologie MeSH
- hornictví MeSH
- molekulární sekvence - údaje MeSH
- RNA ribozomální 16S genetika MeSH
- Streptomyces klasifikace genetika izolace a purifikace metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- antibakteriální látky MeSH
- DNA bakterií MeSH
- RNA ribozomální 16S MeSH
The genetic diversity of streptomycetes in colliery spoil heaps (Sokolov, Czech Republic) was investigated by restriction pattern analysis of 16S-internal transcribed spacer rDNA and 16S sequences. We sampled freshly excavated Miocene sediment (17-19-million-year-old) and four sites of primary succession (initial, early, middle, and late stages; aged 1-44 years) on the same sediment. Active bacteria were present even in fresh Miocene sediment, and the relative proportion of actinomycetes among total bacterial and their genetic diversity increased significantly with the age of the sampling site. The replacement of pioneer species by late succession species during succession was observed. Plate assays of Streptomyces strains revealed 27% antibiotic-producing strains. Screening for nonribosomal peptide synthases and type I polyketide synthases systems suggested that 90% and 55% streptomycetes, respectively, are putative producers of biologically active compounds. The frequencies of tetracycline-, amoxicillin-, and chloramphenicol-resistant streptomycetes were 6%, 9%, and 15%, respectively. These findings document the occurrence of genetic elements encoding antibiotic resistance genes and the production of antibiotics by streptomycetes located in pristine environments. Our results indicate key roles for ancient streptomycetes related to S. microflavus, S. spororaveus, and S. flavofuscus in pioneering community development in freshly excavated substrates.
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GENBANK
EU098010, EU098011, EU098012, EU098013, EU098014, EU098015, EU098016, EU098017, EU098018, EU098019, EU098020, EU098021, EU098022, EU098023, EU098024, EU098025, EU098026, EU098027, EU098028, EU098029, EU098030, EU098031, EU098032, EU098033, EU098034, EU098035, EU098036, EU098037, EU098038, EU098039, EU098040, EU098041, EU098042, EU098043, EU098044, EU098045
