The effect of different molecular weight hyaluronan on macrophage physiology
Jazyk angličtina Země Švédsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
20027154
PII: NEL300709A17
Knihovny.cz E-zdroje
- MeSH
- aktivace makrofágů účinky léků fyziologie MeSH
- antigeny CD44 metabolismus MeSH
- buněčné linie MeSH
- kyselina hyaluronová chemie metabolismus farmakologie MeSH
- lipopolysacharidy toxicita MeSH
- makrofágy chemie účinky léků fyziologie MeSH
- molekulová hmotnost MeSH
- myši MeSH
- NF-kappa B metabolismus MeSH
- oxid dusnatý metabolismus MeSH
- průtoková cytometrie MeSH
- synthasa oxidu dusnatého, typ II metabolismus MeSH
- TNF-alfa metabolismus MeSH
- toll-like receptor 2 metabolismus MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antigeny CD44 MeSH
- Cd44 protein, mouse MeSH Prohlížeč
- kyselina hyaluronová MeSH
- lipopolysacharidy MeSH
- NF-kappa B MeSH
- Nos2 protein, mouse MeSH Prohlížeč
- oxid dusnatý MeSH
- synthasa oxidu dusnatého, typ II MeSH
- Tlr2 protein, mouse MeSH Prohlížeč
- TNF-alfa MeSH
- toll-like receptor 2 MeSH
OBJECTIVES: Hyaluronan, a linear glycosaminoglycan, is an abundant component of extracellular matrix. In its native form, the high-molar-mass hyaluronan polymers have an array of structural and regulatory, mainly anti-inflammatory and anti-angiogenic, functions. In contradiction, the biological effects of fragmented low molecular weight hyaluronan are suggested to be pro-angiogenic and pro-inflammatory. METHODS: The effects of highly purified pharmacological grade hyaluronan of defined molecular weights 11, 52, 87, 250 and 970 kilodaltons were tested on mouse macrophage cell lines RAW 264.7 and MHS. The surface expression of CD44 and Toll-like receptor 2, surface receptors for hyaluronan, was determined by flow cytometry. Activation of macrophages was determined based on nitric oxide and tumour necrosis factor alpha production, inducible nitric oxide synthase expression, and the activation of the nuclear factor kappa B transcriptional factor. RESULTS: Both macrophage cell lines expressed CD44 and Toll-like receptor 2, which were significantly increased by the pre-treatment of macrophages with bacterial lipopolysaccharide. Hyaluronan of any molecular weight did not activate production of nitric oxide or tumour necrosis factor alpha in any mouse macrophage cell lines. Correspondingly, hyaluronan of any tested molecular weight did not stimulate nuclear factor kappa B activation. Similarly, hyaluronan of any molecular weight neither exerted stimulatory nor inhibitory effects on macrophages pre-treated by lipopolysaccharide. CONCLUSION: Interestingly, the data does not support the current view of low molecular weight hyaluronan as a pro-inflammatory mediator for macrophages. Further studies are necessary to clarify the effects of different molecular weight hyaluronan on phagocytes.
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The potency of hyaluronan of different molecular weights in the stimulation of blood phagocytes
