Extracellular enzyme production in Metarhizium anisopliae isolates
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Enzymes metabolism MeSH
- Fungal Proteins metabolism MeSH
- Genetic Variation MeSH
- Insecta microbiology MeSH
- Metarhizium enzymology isolation & purification MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Enzymes MeSH
- Fungal Proteins MeSH
Extracellular enzymes produced by Metarhizium anisopliae are believed to play a key role in cuticle hydrolysis. The in-vitro production of cuticle-degrading enzymes, such as chitinase, proteinase, caseinase, lipase and amylase in fourteen isolates of M. anisopliae exhibited significant natural isolate variability. The isolates were also evaluated for chitinase and proteinase enzyme assays in order to quantify the enzyme production. The growth characteristics and colony morphology of the isolates showed variation and few isolates formed sectors and the colonies were either fluffy or powdery. Among the isolates studied, isolate UM2 was found to show good consistence with the results on enzyme measurements as well as the growth characteristics and colony morphology. Such characterization of isolate variability could rationally be used in the selection of isolates for the production of improved myco-pesticides in the integrated pest management programs.
See more in PubMed
J Biol Chem. 1955 Dec;217(2):959-66 PubMed
FEMS Microbiol Lett. 2002 Aug 6;213(2):251-5 PubMed
J Bacteriol. 1992 Nov;174(22):7398-406 PubMed
J Invertebr Pathol. 2004 Jan;85(1):18-24 PubMed
Appl Microbiol Biotechnol. 2003 Jun;61(5-6):413-23 PubMed
Microbiology (Reading). 1997 Jun;143 ( Pt 6):1983-1992 PubMed
Trends Microbiol. 1996 May;4(5):197-203 PubMed
Appl Environ Microbiol. 2005 Jan;71(1):363-70 PubMed
Appl Environ Microbiol. 2002 Dec;68(12):6383-7 PubMed
J Gen Physiol. 1947 Mar 20;30(4):291-310 PubMed
J Biochem Biophys Methods. 2007 Jun 10;70(4):683-4 PubMed
Comp Biochem Physiol C Toxicol Pharmacol. 2007 Jul-Aug;146(1-2):124-137 PubMed
