Simultaneous genotyping of microsatellite variations in HMOX1 and UGT1A1 genes using multicolored capillary electrophoresis
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20152821
DOI
10.1016/j.clinbiochem.2010.01.006
PII: S0009-9120(10)00031-7
Knihovny.cz E-resources
- MeSH
- Electrophoresis, Capillary methods MeSH
- Genotype MeSH
- Glucuronosyltransferase genetics MeSH
- Heme Oxygenase-1 genetics MeSH
- Polymorphism, Single Nucleotide genetics MeSH
- Humans MeSH
- Microsatellite Repeats genetics MeSH
- Reproducibility of Results MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Glucuronosyltransferase MeSH
- Heme Oxygenase-1 MeSH
- UGT1A1 enzyme MeSH Browser
OBJECTIVES: Our aim was to establish a reliable, rapid, and inexpensive method for the simultaneous genotyping of the HMOX-1 (heme oxygenase-1) and UGT1A1 (bilirubin UDP-glucuronosyltransferase) gene promoter variations. RESULTS: The HMOX1 (GT)(n) and UGT1A1 (TA)(n) gene promoter variations were determined by fragment analysis using a single duplex PCR, with different fluorescent dye-labeled primers; followed by multicolored capillary electrophoresis. CONCLUSION: This novel method provides simultaneous genotyping of key tandem repeat variations in the HMOX1 and UGT1A1 promoters.
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