Mosquito (Diptera: Culicidae) surveillance for arboviruses in an area endemic for West Nile (Lineage Rabensburg) and Tahyna viruses in Central Europe
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20496595
DOI
10.1603/me09219
Knihovny.cz E-resources
- MeSH
- Arboviruses genetics isolation & purification MeSH
- Culex virology MeSH
- Culicidae virology MeSH
- DNA Primers MeSH
- Polymorphism, Single Nucleotide MeSH
- Mice virology MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- RNA, Viral genetics isolation & purification MeSH
- Amino Acid Substitution MeSH
- Viral Proteins genetics MeSH
- West Nile virus genetics isolation & purification MeSH
- Encephalitis Virus, California genetics isolation & purification MeSH
- West Nile Fever mortality veterinary MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice virology MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- DNA Primers MeSH
- RNA, Viral MeSH
- Viral Proteins MeSH
Six viral isolates were obtained from 23,243 female mosquitoes (examined in 513 pools) belonging to 16 species and collected along the lower reaches of the Dyje River in South Moravia (Czech Republic, central Europe) during 2006-2008: five isolates of Orthobunyavirus Tahyna (TAHV, California group, family Bunyaviridae: three isolations from Aedes vexans (Meigen), one from Ae. sticticus (Meigen), one from Culex modestus Ficalbi); and one isolation of Flavivirus West Nile (WNV, Japanese encephalitis group, family Flaviviridae)-strain Rabensburg (proposed lineage 3 of WNV) from Ae. rossicus (Dolbeshkin et al). All viral isolates were recovered from mosquitoes collected in 2006 (15,882 mosquitoes examined), while no virus was isolated from mosquitoes trapped in 2007 and 2008, when 1,555 and 5,806 mosquitoes were examined, respectively. The population density of local mosquitoes was very low in 2007 and 2008 because of warm and dry summer including a considerably low water table, compared with environmental conditions favorable for mosquito development in 2006. The virus isolation procedure was based on intracerebral inoculation of newborn mice. In parallel, more than one-third of the samples (183 pools consisting of 8,470 individual mosquitoes) were also examined by inoculating Vero cell cultures in Leighton tubes. However, the latter method detected only three of the six virus isolates (including WNV-Rabensburg). Ae. rossicus is a new potential vector for WNV-Rabensburg. This species feeds mostly on mammals including man; this raises the question whether this virus lineage is not adapted to an alternative mosquito-mammal cycle in the South-Moravian natural focus.
References provided by Crossref.org
History of Arbovirus Research in the Czech Republic
Cryptic species Anopheles daciae (Diptera: Culicidae) found in the Czech Republic and Slovakia
West Nile virus in overwintering mosquitoes, central Europe
Co-circulation of Usutu virus and West Nile virus in a reed bed ecosystem
