Reciprocal and multi-species chromosome BAC painting in crucifers (Brassicaceae)
Language English Country Switzerland Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
20501976
DOI
10.1159/000312951
PII: 000312951
Knihovny.cz E-resources
- MeSH
- Arabidopsis classification genetics MeSH
- Brassicaceae classification genetics MeSH
- Chromosomes, Plant genetics MeSH
- Species Specificity MeSH
- In Situ Hybridization, Fluorescence MeSH
- Karyotyping MeSH
- Chromosome Painting methods MeSH
- Chromosomes, Artificial, Bacterial genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
In crucifer cytogenomics, BAC contigs of Arabidopsis thaliana have been used as probes for comparative chromosome painting among species. Here we successfully tested chromosome-specific BAC contigs of A. thaliana (n = 5) and A. halleri (n = 8) as probes for reciprocal BAC painting. Furthermore, BAC contigs of both Arabidopsis species were applied as multi-species painting probes to a third crucifer species, Noccaea caerulescens (n = 7), revealing their shared chromosome homeology. Specifically, we found homeology across portions of chromosomes At2, Ah4, and Nc4, which reflects their shared common origin with chromosome AK4 of the Ancestral Crucifer Karyotype (n = 8). We argue that multi-species and multi-directional painting will significantly expedite comparative cytogenomics in Brassicaceae and other plant families.
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