Xylanases of anaerobic fungus Anaeromyces mucronatus
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Anaerobiosis MeSH
- Cellobiose metabolism MeSH
- Cellulose metabolism MeSH
- Fungal Proteins biosynthesis MeSH
- Glycoside Hydrolases biosynthesis chemistry classification MeSH
- Culture Media chemistry MeSH
- Molecular Weight MeSH
- Neocallimastigales enzymology physiology MeSH
- Xylans metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cellobiose MeSH
- Cellulose MeSH
- Fungal Proteins MeSH
- Glycoside Hydrolases MeSH
- Culture Media MeSH
- Xylans MeSH
The anaerobic fungus Anaeromyces mucronatus KF8 grown in batch culture on M10 medium with rumen fluid and microcrystalline cellulose as carbon source produced a broad range of enzymes requisite for degradation of plant structural and storage saccharides including cellulase, endoglucanase, xylanase, alpha-xylosidase, beta-xylosidase, alpha-glucosidase, beta-glucosidase, beta-galactosidase, mannosidase, cellobiohydrolase, amylase, laminarinase, pectinase and pectate lyase. These enzymes were detected in both the intra- and extracellular fractions, but production into the medium was prevalent with the exception of intracellular beta-xylosidase, chitinases, N-acetylglucosaminidase, and lipase. Xylanase activity was predominant among the polysaccharide hydrolases. Extracellular production of xylanase was stimulated by the presence of cellobiose and oat spelt xylan. Zymogram of xylanases of strain KF8 grown on different carbon sources revealed several isoforms of xylanases with approximate molar masses ranging from 26 to 130 kDa.
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