Transcription-dependent rearrangements of actin and nuclear myosin I in the nucleolus
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Actins metabolism MeSH
- Cell Nucleolus metabolism MeSH
- Transcription, Genetic physiology MeSH
- HeLa Cells MeSH
- Immunohistochemistry MeSH
- Humans MeSH
- Myosin Type I metabolism MeSH
- DNA, Ribosomal metabolism MeSH
- RNA, Ribosomal metabolism MeSH
- RNA Polymerase I metabolism MeSH
- Pol1 Transcription Initiation Complex Proteins metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Actins MeSH
- Myosin Type I MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal MeSH
- RNA Polymerase I MeSH
- transcription factor UBF MeSH Browser
- Pol1 Transcription Initiation Complex Proteins MeSH
Nuclear actin and nuclear myosin I (NMI) are important players in transcription of ribosomal genes. Transcription of rDNA takes place in highly organized intranuclear compartment, the nucleolus. In this study, we characterized the localization of these two proteins within the nucleolus of HeLa cells with high structural resolution by means of electron microscopy and gold-immunolabeling. We demonstrate that both actin and NMI are localized in specific compartments within the nucleolus, and the distribution of NMI is transcription-dependent. Moreover, a pool of NMI is present in the foci containing nascent rRNA transcripts. Actin, in turn, is present both in transcriptionally active and inactive regions of the nucleolus and colocalizes with RNA polymerase I and UBF. Our data support the involvement of actin and NMI in rDNA transcription and point out to other functions of these proteins in the nucleolus, such as rRNA maturation and maintenance of nucleolar architecture.
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