Nuclear actin and myosin I are required for RNA polymerase I transcription
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.
Grantová podpora
GM 59648
NIGMS NIH HHS - United States
PubMed
15558034
DOI
10.1038/ncb1190
PII: ncb1190
Knihovny.cz E-zdroje
- MeSH
- aktiny metabolismus MeSH
- buněčné jádro genetika metabolismus MeSH
- buněčné linie MeSH
- genetická transkripce genetika MeSH
- kinasy ribozomálního proteinu S6 MeSH
- lidé MeSH
- myosin typu I metabolismus MeSH
- nádorové buněčné linie MeSH
- proteinkinasy genetika metabolismus MeSH
- protilátky farmakologie MeSH
- rekombinantní fúzní proteiny genetika metabolismus MeSH
- ribozomální DNA genetika MeSH
- RNA interference MeSH
- RNA ribozomální biosyntéza genetika MeSH
- RNA-polymerasa I biosyntéza genetika MeSH
- serin metabolismus MeSH
- transkripční faktory genetika metabolismus MeSH
- transkripční iniciační komplex Pol1 - proteiny MeSH
- vazba proteinů genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Názvy látek
- aktiny MeSH
- kinasy ribozomálního proteinu S6 MeSH
- myosin typu I MeSH
- proteinkinasy MeSH
- protilátky MeSH
- rekombinantní fúzní proteiny MeSH
- ribozomální DNA MeSH
- RNA ribozomální MeSH
- RNA-polymerasa I MeSH
- RRN3 protein, human MeSH Prohlížeč
- serin MeSH
- transkripční faktory MeSH
- transkripční iniciační komplex Pol1 - proteiny MeSH
The presence of actin and nuclear myosin I (NMI) in the nucleus suggests a role for these motor proteins in nuclear functions. We have investigated the role of actin and nuclear myosin I (NMI) in the transcription of ribosomal RNA genes (rDNA). Both proteins are associated with rDNA and are required for RNA polymerase I (Pol I) transcription. Microinjection of antibodies against actin or NMI, as well as short interfering RNA-mediated depletion of NMI, decreased Pol I transcription in vivo, whereas overexpression of NMI augmented pre-rRNA synthesis. In vitro, recombinant NMI activated Pol I transcription, and antibodies to NMI or actin inhibited Pol I transcription both on naked DNA and pre-assembled chromatin templates. Whereas actin associated with Pol I, NMI bound to Pol I through the transcription-initiation factor TIF-IA. The association with Pol I requires phosphorylation of TIF-IA at Ser 649 by RSK kinase, indicating a role for NMI in the growth-dependent regulation of rRNA synthesis.
Citace poskytuje Crossref.org
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