Nuclear actin and myosin I are required for RNA polymerase I transcription
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.
Grant support
GM 59648
NIGMS NIH HHS - United States
PubMed
15558034
DOI
10.1038/ncb1190
PII: ncb1190
Knihovny.cz E-resources
- MeSH
- Actins metabolism MeSH
- Cell Nucleus genetics metabolism MeSH
- Cell Line MeSH
- Transcription, Genetic genetics MeSH
- Ribosomal Protein S6 Kinases MeSH
- Humans MeSH
- Myosin Type I metabolism MeSH
- Cell Line, Tumor MeSH
- Protein Kinases genetics metabolism MeSH
- Antibodies pharmacology MeSH
- Recombinant Fusion Proteins genetics metabolism MeSH
- DNA, Ribosomal genetics MeSH
- RNA Interference MeSH
- RNA, Ribosomal biosynthesis genetics MeSH
- RNA Polymerase I biosynthesis genetics MeSH
- Serine metabolism MeSH
- Transcription Factors genetics metabolism MeSH
- Pol1 Transcription Initiation Complex Proteins MeSH
- Protein Binding genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Names of Substances
- Actins MeSH
- Ribosomal Protein S6 Kinases MeSH
- Myosin Type I MeSH
- Protein Kinases MeSH
- Antibodies MeSH
- Recombinant Fusion Proteins MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal MeSH
- RNA Polymerase I MeSH
- RRN3 protein, human MeSH Browser
- Serine MeSH
- Transcription Factors MeSH
- Pol1 Transcription Initiation Complex Proteins MeSH
The presence of actin and nuclear myosin I (NMI) in the nucleus suggests a role for these motor proteins in nuclear functions. We have investigated the role of actin and nuclear myosin I (NMI) in the transcription of ribosomal RNA genes (rDNA). Both proteins are associated with rDNA and are required for RNA polymerase I (Pol I) transcription. Microinjection of antibodies against actin or NMI, as well as short interfering RNA-mediated depletion of NMI, decreased Pol I transcription in vivo, whereas overexpression of NMI augmented pre-rRNA synthesis. In vitro, recombinant NMI activated Pol I transcription, and antibodies to NMI or actin inhibited Pol I transcription both on naked DNA and pre-assembled chromatin templates. Whereas actin associated with Pol I, NMI bound to Pol I through the transcription-initiation factor TIF-IA. The association with Pol I requires phosphorylation of TIF-IA at Ser 649 by RSK kinase, indicating a role for NMI in the growth-dependent regulation of rRNA synthesis.
References provided by Crossref.org
Plasma membrane and nuclear phosphatidylinositol 4,5-bisphosphate signalling in cancer
Lamin A/C and PI(4,5)P2-A Novel Complex in the Cell Nucleus
Envisioning a role for nuclear actin in prophase I spermatocytes
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Simultaneous detection of multiple targets for ultrastructural immunocytochemistry
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Transcription-dependent rearrangements of actin and nuclear myosin I in the nucleolus
Actin complexes in the cell nucleus: new stones in an old field
Structure and epigenetics of nucleoli in comparison with non-nucleolar compartments
Ultrastructural localization of actin and actin-binding proteins in the nucleus
Nuclear myosin is ubiquitously expressed and evolutionary conserved in vertebrates
Nuclear distribution of actin and myosin I depends on transcriptional activity of the cell
