Nuclear distribution of actin and myosin I depends on transcriptional activity of the cell
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Actins metabolism MeSH
- Cell Nucleolus drug effects metabolism ultrastructure MeSH
- Cell Nucleus drug effects metabolism ultrastructure MeSH
- Phytohemagglutinins pharmacology MeSH
- Transcription, Genetic genetics MeSH
- Humans MeSH
- Lymphocytes drug effects metabolism ultrastructure MeSH
- RNA, Messenger metabolism MeSH
- Myosin Type I metabolism MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Microscopy, Electron, Transmission MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Actins MeSH
- Phytohemagglutinins MeSH
- RNA, Messenger MeSH
- Myosin Type I MeSH
As previous studies suggested, nuclear myosin I (NMI) and actin have important roles in DNA transcription. In this study, we characterized the dynamics of these two proteins during transcriptional activation in phytohemagglutinin (PHA) stimulated human lymphocytes. The stimulation led to strong up-regulation of NMI both on the mRNA and protein level, while actin was relatively stably expressed. The intranuclear distribution of actin and NMI was evaluated using immunogold labeling. In nucleoli of resting cells, actin was localized predominantly to fibrillar centers (FCs), while NMI was located mainly to the dense fibrillar component (DFC). Upon stimulation, FCs remained the main site of actin localization, however, an accumulation of both actin and NMI in the DFC and in the granular component was observed. In the nucleoplasm of resting lymphocytes, both actin and NMI were localized mostly in condensed chromatin. Following stimulation, the majority of both proteins shifted towards the decondensed chromatin. In transcriptionally active cells, both actin and NMI colocalized with nucleoplasmic transcription sites. These results demonstrate that actin and NMI are compartmentalized in the nuclei where they can dynamically translocate depending on transcriptional activity of the cells.
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Mol Biol Cell. 1998 Jun;9(6):1523-36 PubMed
J Struct Biol. 2002 Oct-Dec;140(1-3):3-9 PubMed
Exp Cell Res. 1995 Feb;216(2):285-9 PubMed
Cell. 1998 Nov 25;95(5):625-36 PubMed
J Cell Sci. 1994 Feb;107 ( Pt 2):639-48 PubMed
Nature. 1970 Aug 15;227(5259):680-5 PubMed
Scand J Immunol. 2004 Jun;59(6):566-73 PubMed
J Ultrastruct Res. 1983 Mar;82(3):264-82 PubMed
J Cell Sci. 1999 Oct;112 ( Pt 19):3259-68 PubMed
J Cell Biol. 1996 Apr;133(2):225-34 PubMed
Nucleic Acids Res. 2002 Apr 15;30(8):1725-34 PubMed
Histochem Cell Biol. 2005 Mar;123(3):203-16 PubMed
Histochem Cell Biol. 2000 Mar;113(3):181-7 PubMed
Methods. 2001 Dec;25(4):402-8 PubMed
Biol Cell. 1989;66(3):225-33 PubMed
J Cell Sci. 1997 Sep;110 ( Pt 17):1999-2012 PubMed
Scand J Clin Lab Invest Suppl. 1968;97:77-89 PubMed
Biotechniques. 2000 Aug;29(2):332-7 PubMed
Nature. 1979 Nov 15;282(5736):320-1 PubMed
Biol Cell. 2004 Oct;96(8):555-67 PubMed
Chromosoma. 1995 Feb;103(8):509-16 PubMed
EMBO J. 1998 Mar 16;17 (6):1635-41 PubMed
Curr Opin Cell Biol. 1999 Jun;11(3):385-90 PubMed
Nat Cell Biol. 2004 Nov;6(11):1094-101 PubMed
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2335-9 PubMed
Exp Cell Res. 2004 Mar 10;294(1):140-8 PubMed
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PubMed
Cell. 2002 Feb 22;108(4):513-21 PubMed
J Biol Chem. 1997 Jul 4;272(27):17176-81 PubMed
Biol Cell. 1988;62(3):211-8 PubMed
Genes Cells. 2000 Apr;5(4):289-307 PubMed
J Cell Biol. 1996 Apr;133(2):235-46 PubMed
Trends Cell Biol. 2000 Mar;10(3):92-7 PubMed
Histochem Cell Biol. 2001 Dec;116(6):495-505 PubMed
Z Zellforsch Mikrosk Anat. 1968;92(1):62-9 PubMed
Nat Cell Biol. 2004 Dec;6(12 ):1165-72 PubMed
Trends Cell Biol. 1994 Mar;4(3):86-90 PubMed
Annu Rev Biochem. 2002;71:755-81 PubMed
J Struct Biol. 2000 Dec;132(3):201-10 PubMed
Exp Cell Res. 1985 Oct;160(2):319-30 PubMed
Genes Dev. 2004 Dec 15;18(24):3010-5 PubMed
J Cell Biol. 2001 Apr 2;153(1):229-36 PubMed
Annu Rev Biochem. 2003;72:573-608 PubMed
Eur J Immunol. 1979 Jun;9(6):434-9 PubMed
Histochem Cell Biol. 1998 Feb;109(2):111-8 PubMed
Nat Rev Mol Cell Biol. 2004 May;5(5):410-5 PubMed
Science. 2000 Oct 13;290(5490):337-41 PubMed
Chromosome Res. 2003;11(5):387-401 PubMed
J Ultrastruct Res. 1969 May;27(3):250-65 PubMed
Trends Cell Biol. 2000 May;10 (5):189-96 PubMed
Curr Opin Cell Biol. 2000 Jun;12 (3):372-7 PubMed
J Cell Biol. 2002 May 27;157(5):739-41 PubMed
Cytogenet Cell Genet. 2000;91(1-4):243-52 PubMed
Nat Struct Mol Biol. 2005 Mar;12(3):238-44 PubMed
Exp Cell Res. 1996 Dec 15;229(2):217-25 PubMed
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