Site of transcription of ribosomal RNA and intranucleolar structure in HeLa cells
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
Wellcome Trust - United Kingdom
PubMed
8207086
DOI
10.1242/jcs.107.2.639
Knihovny.cz E-zdroje
- MeSH
- biologické modely MeSH
- buněčné jadérko metabolismus ultrastruktura MeSH
- elektronová mikroskopie MeSH
- genetická transkripce * MeSH
- HeLa buňky MeSH
- lidé MeSH
- permeabilita buněčné membrány MeSH
- RNA ribozomální genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- RNA ribozomální MeSH
Sites of transcription of ribosomal RNA in HeLa cells were visualized by electron microscopy. Cells were either incubated with Br-uridine, or permeabilized and then incubated with BrUTP, before sites containing Br-RNA were immunolabeled with gold particles. Short incubations ensured that most incorporated analogue remained at synthetic sites. Fibrillar centres were unlabelled except at their periphery; label was concentrated over certain regions of the surrounding dense fibrillar component. These results suggest that the dense fibrillar component is the site of rRNA transcription. After dispersing the granular component and the dense fibrillar component by a hypotonic treatment, removal of most chromatin and preparation of resinless sections, fibrillar centres remained fixed to a nucleoskeleton. These structural and functional features are incorporated into a model for rRNA transcription.
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