Site of transcription of ribosomal RNA and intranucleolar structure in HeLa cells
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
Wellcome Trust - United Kingdom
PubMed
8207086
DOI
10.1242/jcs.107.2.639
Knihovny.cz E-resources
- MeSH
- Models, Biological MeSH
- Cell Nucleolus metabolism ultrastructure MeSH
- Microscopy, Electron MeSH
- Transcription, Genetic * MeSH
- HeLa Cells MeSH
- Humans MeSH
- Cell Membrane Permeability MeSH
- RNA, Ribosomal genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- RNA, Ribosomal MeSH
Sites of transcription of ribosomal RNA in HeLa cells were visualized by electron microscopy. Cells were either incubated with Br-uridine, or permeabilized and then incubated with BrUTP, before sites containing Br-RNA were immunolabeled with gold particles. Short incubations ensured that most incorporated analogue remained at synthetic sites. Fibrillar centres were unlabelled except at their periphery; label was concentrated over certain regions of the surrounding dense fibrillar component. These results suggest that the dense fibrillar component is the site of rRNA transcription. After dispersing the granular component and the dense fibrillar component by a hypotonic treatment, removal of most chromatin and preparation of resinless sections, fibrillar centres remained fixed to a nucleoskeleton. These structural and functional features are incorporated into a model for rRNA transcription.
References provided by Crossref.org
Nuclear Phosphoinositides-Versatile Regulators of Genome Functions
Transcription-dependent rearrangements of actin and nuclear myosin I in the nucleolus
Ultrastructural localization of actin and actin-binding proteins in the nucleus
Nuclear distribution of actin and myosin I depends on transcriptional activity of the cell