Electrochemical characterization of repaglinide and its determination in human plasma using liquid chromatography with dual-channel coulometric detection
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
21036677
DOI
10.1016/j.jchromb.2010.09.029
PII: S1570-0232(10)00616-1
Knihovny.cz E-zdroje
- MeSH
- elektrochemické techniky metody MeSH
- hypoglykemika krev chemie MeSH
- karbamáty krev chemie MeSH
- kolorimetrie metody MeSH
- koncentrace vodíkových iontů MeSH
- lidé MeSH
- lineární modely MeSH
- piperidiny krev chemie MeSH
- reprodukovatelnost výsledků MeSH
- rosiglitazon MeSH
- senzitivita a specificita MeSH
- thiazolidindiony analýza MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- hypoglykemika MeSH
- karbamáty MeSH
- piperidiny MeSH
- repaglinide MeSH Prohlížeč
- rosiglitazon MeSH
- thiazolidindiony MeSH
A simple, fast and sensitive HPLC method employing dual-channel coulometric detection for the determination of repaglinide in human plasma is presented. The assay involved extraction of repaglinide by ethyl acetate and isocratic reversed-phase liquid chromatography with dual-channel coulometric detection. The mobile phase composition was 50mM disodium hydrogen phosphate/acetonitrile (60:40, v/v), pH of the mobile phase 7.5 set up with phosphoric acid. For all analyses, the first cell working potential was +380mV, the second was +750mV (vs. Pd/H(2)). Calibration curve was linear over the concentration range of 5-500nmolL(-1). Rosiglitazone was used as an internal standard. The limit of detection (LOD) was established at 2.8nmolL(-1), and the lower limit of quantification (LLOQ) at 8.5nmolL(-1). The developed method was applied to human plasma samples spiked with repaglinide at therapeutical concentrations. It was confirmed that the method is suitable for pharmacokinetic studies or therapeutic monitoring.
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