Electrochemical characterization of repaglinide and its determination in human plasma using liquid chromatography with dual-channel coulometric detection
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21036677
DOI
10.1016/j.jchromb.2010.09.029
PII: S1570-0232(10)00616-1
Knihovny.cz E-resources
- MeSH
- Electrochemical Techniques methods MeSH
- Hypoglycemic Agents blood chemistry MeSH
- Carbamates blood chemistry MeSH
- Colorimetry methods MeSH
- Hydrogen-Ion Concentration MeSH
- Humans MeSH
- Linear Models MeSH
- Piperidines blood chemistry MeSH
- Reproducibility of Results MeSH
- Rosiglitazone MeSH
- Sensitivity and Specificity MeSH
- Thiazolidinediones analysis MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Hypoglycemic Agents MeSH
- Carbamates MeSH
- Piperidines MeSH
- repaglinide MeSH Browser
- Rosiglitazone MeSH
- Thiazolidinediones MeSH
A simple, fast and sensitive HPLC method employing dual-channel coulometric detection for the determination of repaglinide in human plasma is presented. The assay involved extraction of repaglinide by ethyl acetate and isocratic reversed-phase liquid chromatography with dual-channel coulometric detection. The mobile phase composition was 50mM disodium hydrogen phosphate/acetonitrile (60:40, v/v), pH of the mobile phase 7.5 set up with phosphoric acid. For all analyses, the first cell working potential was +380mV, the second was +750mV (vs. Pd/H(2)). Calibration curve was linear over the concentration range of 5-500nmolL(-1). Rosiglitazone was used as an internal standard. The limit of detection (LOD) was established at 2.8nmolL(-1), and the lower limit of quantification (LLOQ) at 8.5nmolL(-1). The developed method was applied to human plasma samples spiked with repaglinide at therapeutical concentrations. It was confirmed that the method is suitable for pharmacokinetic studies or therapeutic monitoring.
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