Surface plasmon resonance biosensor for rapid label-free detection of microribonucleic acid at subfemtomole level
Language English Country United States Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
Grant support
P50 GM076547
NIGMS NIH HHS - United States
P50 GM076547-03
NIGMS NIH HHS - United States
GM076547
NIGMS NIH HHS - United States
PubMed
21090631
PubMed Central
PMC3021551
DOI
10.1021/ac102131s
Knihovny.cz E-resources
- MeSH
- Biosensing Techniques methods MeSH
- DNA MeSH
- Nucleic Acid Hybridization immunology MeSH
- Liver chemistry MeSH
- Limit of Detection MeSH
- Methods MeSH
- MicroRNAs analysis MeSH
- Mice MeSH
- Surface Plasmon Resonance methods MeSH
- Antibodies MeSH
- RNA MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, N.I.H., Extramural MeSH
- Names of Substances
- DNA MeSH
- MicroRNAs MeSH
- Antibodies MeSH
- RNA MeSH
Microribonucleic acids (miRNAs) have been linked with various regulatory functions and disorders, such as cancers and heart diseases. They, therefore, present an important target for detection technologies for future medical diagnostics. We report here a novel method for rapid and sensitive miRNA detection and quantitation using surface plasmon resonance (SPR) sensor technology and a DNA*RNA antibody-based assay. The approach takes advantage of a novel high-performance portable SPR sensor instrument for spectroscopy of surface plasmons based on a special diffraction grating called a surface plasmon coupler and disperser (SPRCD). The surface of the grating is functionalized with thiolated DNA oligonucleotides which specifically capture miRNA from a liquid sample without amplification. Subsequently, an antibody that recognizes DNA*RNA hybrids is introduced to bind to the DNA*RNA complex and enhance sensor response to the captured miRNA. This approach allows detection of miRNA in less than 30 min at concentrations down to 2 pM with an absolute amount at high attomoles. The methodology is evaluated for analysis of miRNA from mouse liver tissues and is found to yield results which agree well with those provided by the quantitative polymerase chain reaction (qPCR).
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