High-throughput SPR sensor for food safety

. 2009 Jan 01 ; 24 (5) : 1399-404. [epub] 20080815

Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic

Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid18809310
Odkazy

PubMed 18809310
DOI 10.1016/j.bios.2008.08.012
PII: S0956-5663(08)00450-8
Knihovny.cz E-zdroje

High-throughput surface plasmon resonance (SPR) biosensor for rapid and parallelized detection of nucleic acids identifying specific bacterial pathogens is reported. The biosensor consists of a high-performance SPR imaging sensor with polarization contrast and internal referencing (refractive index resolution 2 x 10(-7) RIU) and an array of DNA probes microspotted on the surface of the SPR sensor. It is demonstrated that short sequences of nucleic acids (20-23 bases) characteristic for bacterial pathogens such as Brucella abortus, Escherichia coli, and Staphylococcus aureus can be detected at 100 pM levels. Detection of specific DNA or RNA sequences can be performed in less than 15 min by the reported SPR sensor.

Citace poskytuje Crossref.org

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