High-throughput SPR sensor for food safety
Language English Country England, Great Britain Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
18809310
DOI
10.1016/j.bios.2008.08.012
PII: S0956-5663(08)00450-8
Knihovny.cz E-resources
- MeSH
- Bacteria, Aerobic genetics isolation & purification MeSH
- Food Analysis methods MeSH
- Equipment Failure Analysis MeSH
- Biosensing Techniques instrumentation methods MeSH
- Equipment Design MeSH
- DNA, Bacterial analysis genetics MeSH
- Food Contamination analysis MeSH
- Food Microbiology * MeSH
- Surface Plasmon Resonance instrumentation methods MeSH
- Reproducibility of Results MeSH
- Oligonucleotide Array Sequence Analysis instrumentation MeSH
- Sensitivity and Specificity MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Bacterial MeSH
High-throughput surface plasmon resonance (SPR) biosensor for rapid and parallelized detection of nucleic acids identifying specific bacterial pathogens is reported. The biosensor consists of a high-performance SPR imaging sensor with polarization contrast and internal referencing (refractive index resolution 2 x 10(-7) RIU) and an array of DNA probes microspotted on the surface of the SPR sensor. It is demonstrated that short sequences of nucleic acids (20-23 bases) characteristic for bacterial pathogens such as Brucella abortus, Escherichia coli, and Staphylococcus aureus can be detected at 100 pM levels. Detection of specific DNA or RNA sequences can be performed in less than 15 min by the reported SPR sensor.
References provided by Crossref.org
Combined SPRi Sensor for Simultaneous Detection of Nitrate and Ammonium in Wastewater
